Escherichia coli O157:H7 induces attaching-effacing lesions in large intestinal mucosal explants from adult cattle

被引:30
作者
Baehler, AA [1 ]
Moxley, RA [1 ]
机构
[1] Univ Nebraska, Inst Agr & Nat Resources, Dept Vet & Biomed Sci, Lincoln, NE 68583 USA
关键词
attaching-effacing; cattle; large intestine; explant; Escherichia coli O157 : H7;
D O I
10.1111/j.1574-6968.2000.tb09068.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Attaching-effacing (A/E) lesions following natural and experimental infection with Escherichia coli O157:H7 have been seen in neonatal and 3-4-month-old weanling but not older cattle. To test the hypothesis that the adult bovine large intestinal epithelium is resistant to the development of A/E lesions, colonic and rectal mucosal tissue explants from 18-month-old steers were inoculated with E. coli O157:H7 and examined. Epithelial cells of inoculated explants developed A/E lesions at the bacterial attachment sites, providing evidence that the large intestinal mucosal epithelium may be a site of infection that contributes to carriage of E. coli O157:H7 in adult cattle. (C) 2000 Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:239 / 242
页数:4
相关论文
共 13 条
  • [1] Emerging foodborne pathogens: Escherichia coli O157:H7 as a model of entry of a new pathogen into the food supply of the developed world
    Armstrong, GL
    Hollingsworth, J
    Morris, JG
    [J]. EPIDEMIOLOGIC REVIEWS, 1996, 18 (01) : 29 - 51
  • [2] Duration of detection of fecal excretion of Escherichia coli O157:H7 in cattle
    Besser, TE
    Hancock, DD
    Pritchett, LC
    McRae, EM
    Rice, DH
    Tarr, PI
    [J]. JOURNAL OF INFECTIOUS DISEASES, 1997, 175 (03) : 726 - 729
  • [3] Experimental Escherichia coli O157:H7 carriage in calves
    Brown, CA
    Harmon, BG
    Zhao, T
    Doyle, MP
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (01) : 27 - 32
  • [4] EXPERIMENTAL-INFECTION OF CALVES AND ADULT CATTLE WITH ESCHERICHIA-COLI O157-H7
    CRAY, WC
    MOON, HW
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (04) : 1586 - 1590
  • [5] Dean-Nystrom E. A., 1999, P51
  • [6] Dean-Nystrom EA, 1998, INFECT IMMUN, V66, P4560
  • [7] Enterohemorrhagic Escherichia coli O157:H7 produces Tir, which is translocated to the host cell membrane but is not tyrosine phosphorylated
    DeVinney, R
    Stein, M
    Reinscheid, D
    Abe, A
    Ruschkowski, S
    Finlay, BB
    [J]. INFECTION AND IMMUNITY, 1999, 67 (05) : 2389 - 2398
  • [8] Prevalence of Escherichia coli O157:H7 in range beef calves at weaning
    Laegreid, WW
    Elder, RO
    Keen, JE
    [J]. EPIDEMIOLOGY AND INFECTION, 1999, 123 (02) : 291 - 298
  • [9] ENTEROHEMORRHAGIC ESCHERICHIA-COLI O157-H7 REQUIRES INTIMIN TO COLONIZE THE GNOTOBIOTIC PIG INTESTINE AND TO ADHERE TO HEP-2 CELLS
    MCKEE, ML
    MELTONCELSA, AR
    MOXLEY, RA
    FRANCIS, DH
    OBRIEN, AD
    [J]. INFECTION AND IMMUNITY, 1995, 63 (09) : 3739 - 3744
  • [10] NIETFELD JC, 1991, AM J VET RES, V52, P1142