Field evaluation of a novel colorimetric method -: Double-site enzyme-linked lactate dehydrogenase immunodetection assay -: To determine drug susceptibilities of Plasmodium falciparum clinical isolates from northwestern Thailand

被引：15

作者：

Brockman, A

Singlam, S

Phiaphun, L

Looareesuwan, S

White, NJ

Nosten, F

机构：

[1] Shoklo Malaria Res Unit, Tak 63110, Thailand

[2] Mahidol Univ, Fac Trop Med, Bangkok 10400, Thailand

[3] Univ Queensland, Australian Ctr Int Trop Hlth & Nutr, Brisbane, Qld, Australia

[4] John Radcliffe Hosp, Nuffield Dept Clin Med, Ctr Trop Dis, Oxford, England

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2004年 / 48卷 / 04期

关键词：

D O I：

10.1128/AAC.48.4.1426-1429.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A double-site enzyme-linked lactate dehydrogenase enzyme inummodetection assay was tested against field isolates of Plasmodium falciparum for assessing in vitro drug susceptibilities to a wide range of antimalarial drugs. Its sensitivity allowed the use of parasite densities as low as 200 parasites/mul of blood. Being a nonisotopic, colorimetric assay, it lies within the capabilities of a modest laboratory at the district level.

引用

页码：1426 / 1429

页数：4

共 7 条

[1] Plasmodium falciparum antimalarial drug susceptibility on the north-western border of Thailand during five years of extensive use of artesunate-mefloquine [J].