AMSH regulates calcium-sensing receptor signaling through direct interactions

被引：23

作者：

Herrera-Vigenor, Francisco

Hernandez-Garcia, Ricardo

Valadez-Sanchez, Margarita

Vazquez-Prado, Jose

Reyes-Cruz, Guadalupe

机构：

[1] Inst Politecn Nacl, CINVESTAV, Dept Cell Biol, Mexico City 07000, DF, Mexico

[2] Inst Politecn Nacl, CINVESTAV, Dept Pharmacol, Mexico City 07000, DF, Mexico

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2006年 / 347卷 / 04期

关键词：

calcium-sensing receptor; G-protein-coupled receptors; AMSH; ubiquitin isopeptidase;

D O I：

10.1016/j.bbrc.2006.06.169

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Calcium-sensing receptor (CaR) activates intracellular pathways controlling calcium homeostasis. CaR carboxyl-terminal mutants associated with metabolic diseases suggest that unidentified proteins interact with the carboxyl-terminal region of this receptor. To address this possibility, we screened for CaR-interacting proteins using the carboxyl terminus of CaR (CaR Delta 895-1075 deletion mutant)We identified AMSH, an ubiquitin isopeptidase, as a CaR-interacting partner. AMSH caused a decrease on the signaling properties of wild-type and mutant CaR. Our results indicate that AMSH, which has been recently characterized as a regulator of the endosomal sorting of epidermal growth factor receptor, represents a novel modulator of Calk signaling. (c) 2006 Elsevier Inc. All rights reserved.

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收藏

页码：924 / 930

页数：7

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