Determination of organic acids in leaves and rinds of Garcinia indica (Desr.) by LC

被引：48

作者：

Jayaprakasha, GK ^{[1
]}

Sakariah, KK ^{[1
]}

机构：

[1] Cent Food Technol Res Inst, Mysore 570013, Karnataka, India

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2002年 / 28卷 / 02期

关键词：

Garcinia indica; (-)-hydroxycitric acid; antiobesity;

D O I：

10.1016/S0731-7085(01)00623-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Organic acids in leaves and rinds of Garcinia indica (Kokam) were determined by high-performance liquid chromatography. The major organic acid in leaves and rinds has been found to be (-)-hydroxycitric acid present to the extent of 4.1-4.6 and 10.3-12.7%, respectively, by isocratic elution with 8 mM sulfuric acid as mobile phase with a flow rate of 1.0 ml/min using UV detection at 210 nm. Hydroxycitric acid lactone and citric acid are present in leaves and rinds in minor quantities. This method has been shown to be very reproducible with the coefficient of variation ranging from 2.8 to 4.2%. This is the first report on the composition of organic acids in the leaves and rinds of G. indica by HPLC. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：379 / 384

页数：6