Naive CD8+ T cells do not require costimulation for proliferation and differentiation into cytotoxic effector cells

被引:91
作者
Wang, B
Maile, R
Greenwood, R
Collins, EJ
Frelinger, JA
机构
[1] Univ N Carolina, Dept Microbiol & Immunol, Chapel Hill, NC 27599 USA
[2] Univ N Carolina, Dept Biochem & Biophys, Chapel Hill, NC 27599 USA
关键词
D O I
10.4049/jimmunol.164.3.1216
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Most current models of T cell activation postulate a requirement for two distinct signals. One signal is delivered through the TCR by engagement with peptide/MHC complexes, and the second is delivered by interaction between costimulatory molecules such as CD28 and its ligands CD80 and CD86, Soluble peptide/MHC tetramers provide an opportunity to test whether naive CD8(+) T cells can be activated via the signal generated through the TCR-alpha beta in the absence of any. potential costimulatory molecules. Using T cells from two different TCR transgenic mice in vitro, we find that TCR engagement by peptide/MHC tetramers is sufficient for the activation of naive CD8(+) T cells. Furthermore, these T cells proliferate, produce cytokines, and differentiate into cytolytic effecters. Under the conditions where anti-CD28 is able to enhance proliferation of normal B6 CD4(+), CD8(+), and TCR transgenic CD8(+) T cells with anti-CD3, we see no effect of anti-CD28 on proliferation induced by tetramers, The results of this experiment argue that given a strong signal delivered through the TCR by an authentic ligand, no costimulation is required.
引用
收藏
页码:1216 / 1222
页数:7
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