A novel thermophilic glycosynthase that effects branching glycosylation

被引：70

作者：

Trincone, A

Perugino, G

Rossi, M

Moracci, M

机构：

[1] CNR, Ist Chim Mol Interesse Biol, I-80072 Arco Felice Napoli, Naples, Italy

[2] CNR, Inst Prot Biochem & Enzymol, I-80125 Naples, Italy

[3] Univ Naples Federico II, Dipartimento Chim Organ & Biol, I-80134 Naples, Italy

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2000年 / 10卷 / 04期

关键词：

D O I：

10.1016/S0960-894X(99)00700-3

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

A novel thermophilic glycosynthase that effects branching glycosylation has been obtained by mutation of the nucleophile in the active site of the glycosidase from Sulfolobus solfataricus. Two methods for the use of this mutant are reported. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

收藏

页码：365 / 368

页数：4

相关论文

共 12 条

[1]

BOYER V, 1999, 3 CARB BIOENG M 11 1

[2] Production, properties and applications of food-grade oligosaccharides [J].

Crittenden, RG ;

Playne, MJ .

TRENDS IN FOOD SCIENCE & TECHNOLOGY, 1996, 7 (11) :353-361

[3]

Heightman TD, 1999, ANGEW CHEM INT EDIT, V38, P750, DOI 10.1002/(SICI)1521-3773(19990315)38:6<750::AID-ANIE750>3.0.CO

[4]

2-6

[5] POLYSACCHARIDES IN FUNGI .27. STRUCTURE AND ANTITUMOR-ACTIVITY OF A BRANCHED (1-]3)-BETA-D-GLUCAN FROM THE ALKALINE EXTRACT OF AMANITA-MUSCARIA [J].

KIHO, T ;

KATSURAGAWA, M ;

NAGAI, K ;

UKAI, S ;

HAGA, M .

CARBOHYDRATE RESEARCH, 1992, 224 :237-243

[6] Glycosynthases: Mutant glycosidases for oligosaccharide synthesis [J].

Mackenzie, LF ;

Wang, QP ;

Warren, RAJ ;

Withers, SG .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1998, 120 (22) :5583-5584

[7] From β-glucanase to β-glucansynthase:: glycosyl transfer to α-glycosyl fluorides catalyzed by a mutant endoglucanase lacking its catalytic nucleophile [J].

Malet, C ;

Planas, A .

FEBS LETTERS, 1998, 440 (1-2) :208-212

[8] Restoration of the activity of active-site mutants of the hyperthermophilic β-glycosidase from Sulfolobus solfataricus:: Dependence of the mechanism on the action of external nucleophiles [J].

Moracci, M ;

Trincone, A ;

Perugino, G ;

Ciaramella, M ;

Rossi, M .

BIOCHEMISTRY, 1998, 37 (49) :17262-17270

[9] ENZYMATIC-SYNTHESIS OF POLYOL-GLYCOSIDES AND MASKED POLYOL-GLYCOSIDES USING BETA-GLYCOSIDASE OF SULFOLOBUS-SOLFATARICUS [J].

TRINCONE, A ;

IMPROTA, R ;

GAMBACORTA, A .

BIOCATALYSIS AND BIOTRANSFORMATION, 1995, 12 (01) :77-88

[10] Probing the mechanism of Bacillus 1,3-1,4-β-D-glucan 4-glucanohydrolases by chemical rescue of inactive mutants at catalytically essential residues [J].

Viladot, JL ;

de Ramon, E ;

Durany, O ;

Planas, A .

BIOCHEMISTRY, 1998, 37 (32) :11332-11342