Functional and quantitative proteomics using SILAC

被引：718

作者：

Mann, Matthias ^{[1
]}

机构：

[1] Max Planck Inst Biochem, Dept Proteom & Signal Transduct, D-82152 Martinsried, Germany

来源：

NATURE REVIEWS MOLECULAR CELL BIOLOGY | 2006年 / 7卷 / 12期

关键词：

D O I：

10.1038/nrm2067

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Researchers in many biological areas now routinely characterize proteins by mass spectrometry. Among the many formats for quantitative proteomics, stable-isotope labelling by amino acids in cell culture (SILAC) has emerged as a simple and powerful one. SILAC removes false positives in protein-interaction studies, reveals large-scale kinetics of proteomes and - as a quantitative phosphoproteomics technology - directly uncovers important points in the signalling pathways that control cellular decisions.

引用

页码：952 / 958

页数：8

共 54 条

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