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Development of a RT-nested PCR test detecting pigeon Paramyxovirus-l directly from organs of infected animals
被引:17
作者:
Barbezange, C
[1
]
Jestin, V
[1
]
机构:
[1] U VIPAC, AFSSA Ploufragan, F-22440 Ploufragan, France
关键词:
Paramyxovirus;
pigeon;
RT-PCR;
Newcastle disease;
nucleoprotein gene;
D O I:
10.1016/S0166-0934(02)00148-9
中图分类号:
Q5 [生物化学];
学科分类号:
071010 ;
081704 ;
摘要:
A RT-nested PCR that amplifies part of the conserved nucleoprotein gene of avian Paramyxovirus type I is described. The technique allowed the detection of pigeon Paramyxovirus type 1 (pPMV-1) virus directly from a wide range of infected chicken and pigeon organs, and should be able to detect typical Newcastle disease viruses too. Compared with the reference method, the developed RT-nested PCR was found more sensitive, as it was able to detect virus genome in infected pigeon organs at late stage of infection, when virus isolation failed. Such a molecular technique represents an alternative method of diagnosis for research purposes on pPMV-1 variants, for example to study pathogenesis aspects of the infection or to assess the efficacy of vaccines. (C) 2002 Elsevier Science B.V. All rights reserved.
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页码:197 / 207
页数:11
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