Development of a RT-nested PCR test detecting pigeon Paramyxovirus-l directly from organs of infected animals

被引:17
作者
Barbezange, C [1 ]
Jestin, V [1 ]
机构
[1] U VIPAC, AFSSA Ploufragan, F-22440 Ploufragan, France
关键词
Paramyxovirus; pigeon; RT-PCR; Newcastle disease; nucleoprotein gene;
D O I
10.1016/S0166-0934(02)00148-9
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A RT-nested PCR that amplifies part of the conserved nucleoprotein gene of avian Paramyxovirus type I is described. The technique allowed the detection of pigeon Paramyxovirus type 1 (pPMV-1) virus directly from a wide range of infected chicken and pigeon organs, and should be able to detect typical Newcastle disease viruses too. Compared with the reference method, the developed RT-nested PCR was found more sensitive, as it was able to detect virus genome in infected pigeon organs at late stage of infection, when virus isolation failed. Such a molecular technique represents an alternative method of diagnosis for research purposes on pPMV-1 variants, for example to study pathogenesis aspects of the infection or to assess the efficacy of vaccines. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:197 / 207
页数:11
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