Lab-on-a-chip technologies for stem cell analysis

被引：103

作者：

Ertl, Peter ^{[1
]}

Sticker, Drago ^{[1
]}

Charwat, Verena ^{[2
]}

Kasper, Cornelia ^{[2
]}

Lepperdinger, Guenter ^{[3
]}

机构：

[1] AIT Austrian Inst Technol GmbH, BioSensor Technol, Vienna, Austria

[2] Univ Nat Resources & Life Sci, Dept Biotechnol, Vienna, Austria

[3] Univ Innsbruck, Inst Biomed Aging, Tyrol, Austria

来源：

TRENDS IN BIOTECHNOLOGY | 2014年 / 32卷 / 05期

关键词：

lab-on-a-chip; microfluidics; biosensors; stem cells; SHEAR-STRESS; OSTEOGENIC DIFFERENTIATION; MICROFLUIDIC PLATFORM; CULTURE; COCULTURE; GRADIENT; STRAIN; MATRIX; STIMULATION; CULTIVATION;

D O I：

10.1016/j.tibtech.2014.03.004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

The combination of microfabrication-based technologies with cell biology has laid the foundation for the development of advanced in vitro diagnostic systems capable of analyzing cell cultures under physiologically relevant conditions. In the present review, we address recent lab-on-a-chip developments for stem cell analysis. We highlight in particular the tangible advantages of microfluidic devices to overcome most of the challenges associated with stem cell identification, expansion and differentiation, with the greatest advantage being that lab-on-a-chip technology allows for the precise regulation of culturing conditions, while simultaneously monitoring relevant parameters using embedded sensory systems. State-of-the-art lab-on-a-chip platforms for in vitro assessment of stem cell cultures are presented and their potential future applications discussed.

引用

收藏

页码：245 / 253

页数：9

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