Differentiation of the raw material of the Iberian pig meat industry based on the use of amplified fragment length polymorphism

被引：41

作者：

Alves, E ^{[1
]}

Castellanos, C ^{[1
]}

Olivo, C ^{[1
]}

Silió, L ^{[1
]}

Rodríguez, C ^{[1
]}

机构：

[1] INIA, Dept Mejora Genet & Biotecnol, Madrid 28040, Spain

来源：

MEAT SCIENCE | 2002年 / 61卷 / 02期

关键词：

AFLP; Iberian pig identification;

D O I：

10.1016/S0309-1740(01)00179-6

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The present study is the first step of the development of a new analytical technique to discriminate the genotypes of purebred Iberian and crossbred Iberian x Duroc pigs. A DNA test of breed identity has clear advantages for the protection of the brand name of high-prized products of purebred Iberian pigs. Amplified fragment length polymorphism procedure was used to analyze the nomic DNA samples of 77 Duroc and 80 Iberian pigs from the most representative populations. Thirteen printer combinations produced 588 produced 588 amplified fragments. A total number of 139 fragments were polymorphic and 14 out of them were detected in Duroc and never in Iberian pigs. A panel of the nine most frequent markers allows the discrimination between purebred and crossbred animals. The values of the probability of exclusion of the pure Iberian origin (P-EC) were 0.97 and 0.78, for crossbred individuals with 50 or 25% of Duroc genes, respectively. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：157 / 162

页数：6

共 18 条

[1] AFLP™ markers for DNA fingerprinting in cattle [J].

Ajmone-Marsan, P ;

Valentini, A ;

Cassandro, M ;

Vecchiotti-Antaldi, G ;

Bertoni, G ;

Kuiper, M .

ANIMAL GENETICS, 1997, 28 (06) :418-426

[2] Discriminating among cattle breeds using genetic markers [J].

Blott, SC ;

Williams, JL ;

Haley, CS .

HEREDITY, 1999, 82 (6) :613-619

[3] Genomic identification of the breed of an individual or its tissue [J].

Ciampolini, R ;

Leveziel, H ;

Mazzanti, E ;

Grohs, C ;

Cianci, D .

MEAT SCIENCE, 2000, 54 (01) :35-40

[4]

GIANDINI GC, 1999, GENEBANKS CONSERVATI, P11

[5] The use of amplified fragment length polymorphism (AFLP) in the isolation of sex-specific markers [J].

Griffiths, R ;

Orr, K .

MOLECULAR ECOLOGY, 1999, 8 (04) :671-674

[6] Multicolour fluorescent detection and mapping of AFLP markers in chicken (Gallus domesticus) [J].

Herbergs, J ;

Siwek, M ;

Crooijmans, RPMA ;

Van der Poel, JJ ;

Groenen, MAM .

ANIMAL GENETICS, 1999, 30 (04) :274-285

[7] Application of AFLP markers to genome mapping in poultry [J].

Knorr, C ;

Cheng, HH ;

Dodgson, JB .

ANIMAL GENETICS, 1999, 30 (01) :28-35

[8] Sustained utilization of the Iberian pig breed [J].

Lopez-Bote, CJ .

MEAT SCIENCE, 1998, 49 :S17-S27

[9] A quick and simple method for the identification of meat species and meat products by PCR assay [J].

Matsunaga, T ;

Chikuni, K ;

Tanabe, R ;

Muroya, S ;

Shibata, K ;

Yamada, J ;

Shinmura, Y .

MEAT SCIENCE, 1999, 51 (02) :143-148

[10] IDENTIFICATION OF MARKERS LINKED TO DISEASE-RESISTANCE GENES BY BULKED SEGREGANT ANALYSIS - A RAPID METHOD TO DETECT MARKERS IN SPECIFIC GENOMIC REGIONS BY USING SEGREGATING POPULATIONS [J].

MICHELMORE, RW ;

PARAN, I ;

KESSELI, RV .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (21) :9828-9832

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