ANTIOXIDANT PROPERTIES OF THE EXTRACT AND SUBFRACTIONS FROM OLD LEAVES OF TOONA SINENSIS ROEM (MELIACEAE)

被引：14

作者：

Jiang, Shen-Hua ^{[1
,2
]}

Wang, Chang-Lu ^{[1
]}

Chen, Zhi-Qiang ^{[1
]}

Chen, Mian-Hua ^{[1
]}

Wang, Yu-Rong ^{[1
]}

Liu, Chang-Jin ^{[1
]}

Zhou, Qing-Li ^{[3
]}

Li, Zhen-Jing ^{[1
]}

机构：

[1] Tianjin Univ Sci & Technol, Minist Educ, Key Lab Food Nutr & Safety, Tianjin 300457, Peoples R China

[2] Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Peoples R China

[3] Tianjin Univ Sci & Technol, Tianjin Engn Ctr Food Proc, Tianjin 300457, Peoples R China

来源：

JOURNAL OF FOOD BIOCHEMISTRY | 2009年 / 33卷 / 03期

关键词：

OXIDATIVE STRESS; PLANT-EXTRACTS; CANCER CELLS; STABILIZATION; CAPACITY; RADICALS; HERBS; LEAF; OIL;

D O I：

10.1111/j.1745-4514.2009.00226.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The methanolic crude extract and four subfractions (hexane fraction, ethyl acetate fraction, n-butanol fraction and aqueous fraction) of old leaves of Toona sinensis (L.T.S.) were prepared by sequential partitioning, and their antioxidant activities were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical-scavenging assay, ferric reducing ability of plasma (FRAP) assay, beta-carotene bleaching method and stabilizing soybean oil. The results indicated that old L.T.S. possess powerful antioxidant activity and could prevent oxidation of soybean oil. The ethyl acetate-soluble fraction had the strongest antioxidant activity, with high levels of total flavonoids content (108.57 +/- 4.21 mg rutin equivalents/g), total phenol contents (262.09 +/- 1.93 mg gallic acid equivalents/g), reductive activity (467.53 +/- 6.75 mg ascorbic acid [Vc] equivalents/g), FRAP value (10578.71 +/- 149.00 mu M FRAP/g) and strong DPPH radical scavenging capacity, respectively. These results suggest that the extract of old L.T.S. is a promising natural alternative to synthetic antioxidants as functional food ingredient and a natural antioxidant in soybean oil.

引用

页码：425 / 441

页数：17

共 30 条

[1]

Free-radical scavenging capacity and antioxidant activity of selected plant species from the Canadian prairies [J].