Functional Nucleic Acid Sensors

被引:1854
作者
Liu, Juewen [1 ]
Cao, Zehui [1 ]
Lu, Yi [1 ]
机构
[1] Univ Illinois, Dept Chem, Urbana, IL 61801 USA
基金
美国国家科学基金会;
关键词
IN-VITRO SELECTION; RESONANCE ENERGY-TRANSFER; SINGLE-STRANDED-DNA; NONEQUILIBRIUM CAPILLARY-ELECTROPHORESIS; ROLLING CIRCLE AMPLIFICATION; APTAMER-BASED SENSORS; ENGINEERED ALLOSTERIC RIBOZYMES; FLUORESCENT DNAZYME BIOSENSORS; FREE ELECTROCHEMICAL DETECTION; TYPE-1; REVERSE-TRANSCRIPTASE;
D O I
10.1021/cr030183i
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Functional DNA and RNA sensors, the nucleic acid enzyme (NAE) based sensors, are reviewed. Selection of a UO 2 ion-specific RNA-cleaving DNAzyme is used to illustrate the principle of selection of DNAzymes. It is found that during selection for RNA-cleaving DNAzymes, if a fluorophore and a quencher is incorporated immediately adjacent to the cleavage site, the resulting NAEs are found as sensors after selection. An investigation of Pb 2+-induced disassembly of AuNP aggregates show that the DNAzyme is inactive in head-to-tail aligned aggregates and active in tail-to-tail aligned aggregates. AuNPs are employed as quenchers for designing structure-switching aptamer sensors for protein detection. The performance of the aptamer-based system is comparable to traditional all antibody-based Enzyme-linked immunosorbent assays (ELISA) detections, and a detection limit of 25 ρg/mL is reported.
引用
收藏
页码:1948 / 1998
页数:51
相关论文
共 645 条
[1]   Protein detecting arrays based on cationic polythiophene-DNA-aptamer complexes [J].
Aberem, Maite Bera ;
Najari, Ahmed ;
Ho, Hoang-Anh ;
Gravel, Jean-Francois ;
Nobert, Philippe ;
Boudreau, Denis ;
Leclerc, Mario .
ADVANCED MATERIALS, 2006, 18 (20) :2703-+
[2]   Structure-switching allosteric deoxyribozymes [J].
Achenbach, JC ;
Nutiu, R ;
Li, YF .
ANALYTICA CHIMICA ACTA, 2005, 534 (01) :41-51
[3]   DNAzymes:: From creation in vitro to application in vivo [J].
Achenbach, JC ;
Chiuman, W ;
Cruz, RPG ;
Li, Y .
CURRENT PHARMACEUTICAL BIOTECHNOLOGY, 2004, 5 (04) :321-336
[4]   Selection of ribozymes that catalyse multiple-turnover Diels-Alder cycloadditions by using in vitro compartmentalization [J].
Agresti, JJ ;
Kelly, BT ;
Jäschke, A ;
Griffiths, AD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (45) :16170-16175
[5]   Characterization of pH3DZ1 - An RNA-cleaving deoxyribozyme with optimal activity at pH 3 [J].
Ali, Md. Monsur ;
Kandadai, Srinivas A. ;
Li, Yingfu .
CANADIAN JOURNAL OF CHEMISTRY, 2007, 85 (04) :261-273
[6]   Controlling the rate of organic reactions:: rational design of allosteric Diels-Alderase ribozymes [J].
Amontov, Sergey ;
Jaeschke, Andres .
NUCLEIC ACIDS RESEARCH, 2006, 34 (18) :5032-5038
[7]   DNA aptamers selected against the HIV-1 RNase H display in vitro antiviral activity [J].
Andreola, ML ;
Pileur, F ;
Calmels, C ;
Ventura, M ;
Tarrago-Litvak, L ;
Toulmé, JJ ;
Litvak, S .
BIOCHEMISTRY, 2001, 40 (34) :10087-10094
[8]   Allosteric regulation of a ribozyme activity through ligand-induced conformational change [J].
Araki, M ;
Okuno, Y ;
Hara, Y ;
Sugiura, Y .
NUCLEIC ACIDS RESEARCH, 1998, 26 (14) :3379-3384
[9]   Coupling between substrate binding and allosteric regulation in ribozyme catalysis [J].
Araki, M ;
Hashima, M ;
Okuno, Y ;
Sugiura, Y .
BIOORGANIC & MEDICINAL CHEMISTRY, 2001, 9 (05) :1155-1163
[10]   Aptamers switch on fluorescence of triphenylmethane dyes [J].
Babendure, JR ;
Adams, SR ;
Tsien, RY .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (48) :14716-14717