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Functional Nucleic Acid Sensors
被引:1854
作者:
Liu, Juewen
[1
]
Cao, Zehui
[1
]
Lu, Yi
[1
]
机构:
[1] Univ Illinois, Dept Chem, Urbana, IL 61801 USA
基金:
美国国家科学基金会;
关键词:
IN-VITRO SELECTION;
RESONANCE ENERGY-TRANSFER;
SINGLE-STRANDED-DNA;
NONEQUILIBRIUM CAPILLARY-ELECTROPHORESIS;
ROLLING CIRCLE AMPLIFICATION;
APTAMER-BASED SENSORS;
ENGINEERED ALLOSTERIC RIBOZYMES;
FLUORESCENT DNAZYME BIOSENSORS;
FREE ELECTROCHEMICAL DETECTION;
TYPE-1;
REVERSE-TRANSCRIPTASE;
D O I:
10.1021/cr030183i
中图分类号:
O6 [化学];
学科分类号:
0703 ;
摘要:
Functional DNA and RNA sensors, the nucleic acid enzyme (NAE) based sensors, are reviewed. Selection of a UO 2 ion-specific RNA-cleaving DNAzyme is used to illustrate the principle of selection of DNAzymes. It is found that during selection for RNA-cleaving DNAzymes, if a fluorophore and a quencher is incorporated immediately adjacent to the cleavage site, the resulting NAEs are found as sensors after selection. An investigation of Pb 2+-induced disassembly of AuNP aggregates show that the DNAzyme is inactive in head-to-tail aligned aggregates and active in tail-to-tail aligned aggregates. AuNPs are employed as quenchers for designing structure-switching aptamer sensors for protein detection. The performance of the aptamer-based system is comparable to traditional all antibody-based Enzyme-linked immunosorbent assays (ELISA) detections, and a detection limit of 25 ρg/mL is reported.
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页码:1948 / 1998
页数:51
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