Inhibition of degranulation and interleukin-6 production in mast cells derived from mice deficient in protein kinase Cβ

The antigen-mediated activation of mast cells by means of IgE antibodies bound to the cell surface leads to direct interactions between Fc epsilon RI receptor cytoplasmic domains and various intracellular proteins, These interactions initiate diverse signal-transduction pathways, and the activation of these pathways results in the immediate release of proinflammatory agents. A delayed response also occurs and includes the release of various cytokines, It is clear that the activation of kinases is a requirement for the exocytosis observed in mast cells. In addition to the tyrosine phosphorylation of the affected system by soluble tyrosine kinases, activity of protein kinase C (PKC) results in serine or threonine phosphorylation of multiple protein substrates, In this study, we found that mast cells derived from PKC beta-deficient mice produce less interleukin 6 in response to IgE-Ag. The inhibition of exocytosis in the PKC beta-deficient mast cells occurred whether the stimuli were due to the aggregation of the mast cell surface Fc epsilon RI or to the calcium ionophore, ionomycin. However, no significant changes were observed in the proliferative response of the mast cells to interleukin 3 (IL-3) or in their apoptotic rate after IL-3 depletion. (C) 2000 by The American Society of Hematology.