A note on the physiological background of the ethylene oxide adduct 7-(2-hydroxyethyl)guanine in DNA from human blood

被引：20

作者：

Bolt, HM

Leutbecher, M

Golka, K

机构：

[1] Institut für Arbeitsphysiologie, Universität Dortmund, D-44139 Dortmund

来源：

ARCHIVES OF TOXICOLOGY | 1997年 / 71卷 / 11期

关键词：

ethylene; ethylene oxide; 7-(2-hydroxyethyl)guanine; DNA adducts;

D O I：

10.1007/s002040050451

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

A newly developed high-performance liquid chromatography (HPLC) method involves derivatization with phenylglyoxal and fluorescence detection, using 7-methylguanine as internal standard. The physiological background of the adduct 7-(2-hydroxyethyl)guanine in DNA isolated from human blood was determined by this method. In five persons the range of the adduct was between 2.1 and 5.8 pmol/mg DNA (mean 3.2). This finding is consistent with previous data obtained by others, using different analytical methods, and points to an intrinsic carcinogenic risk due to endogenous ethylene oxide.

引用

页码：719 / 721

页数：3

共 11 条

[1] DOSE-DNA ADDUCT RELATIONSHIP FOR ETHYLENE-OXIDE [J].