A novel method for measurement of diffusion coefficients of proteins and DNA in solution

被引：14

作者：

Baden, N ^{[1
]}

Terazima, M ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Sci, Dept Chem, Kyoto 6068502, Japan

来源：

CHEMICAL PHYSICS LETTERS | 2004年 / 393卷 / 4-6期

关键词：

D O I：

10.1016/j.cplett.2004.06.104

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

A novel method for measurement of the diffusion coefficients (D) of various proteins and DNA in solution using the transient grating method is proposed. The target molecules are labeled by a photo-reactive molecule in dark and the labeled molecule is photo-decomposed by the grating light. This method is demonstrated to be a convenient D measurement with a fast time resolution. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：539 / 545

页数：7

共 26 条

[1]

[Anonymous], 1986, LASER INDUCED DYNAMI

[2] FLUORESCENCE CORRELATION SPECTROSCOPY AND BROWNIAN ROTATIONAL DIFFUSION [J].