Effect of increased vegetable and fruit consumption on markers of oxidative cellular damage

被引:158
作者
Thompson, HJ [1 ]
Heimendinger, J
Haegele, A
Sedlacek, SM
Gillette, C
O'Neill, C
Wolfe, P
Conroy, C
机构
[1] AMC, Canc Res Ctr, Ctr Nutr Prevent Dis, Lakewood, CO 80214 USA
[2] AMC, Canc Res Ctr, Ctr Behav Sci Res, Lakewood, CO 80214 USA
[3] AMC, Canc Res Ctr, Ctr Res Methodol & Biometr, Lakewood, CO 80214 USA
[4] Univ Colorado, Hlth Sci Ctr, Dept Family Med, Denver, CO 80102 USA
关键词
D O I
10.1093/carcin/20.12.2261
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The goal of this study was to test the hypothesis that increased consumption of vegetables and fruit would reduce markers of oxidative cellular damage that can be assessed in blood or urine, Twenty-eight women participated in a 14 day dietary intervention. The primary end-points assessed were: 8-hydroxydeoxyguanosine (8-OHdG) in DNA isolated from peripheral lymphocytes, determined by HPLC with electrochemical detection; 8-OHdG excreted in urine, measured by ELISA; malondialdehyde (MDA) in urine, measured by fluorimetric detection following derivatization with thiobarituric acid and separation via HPLC; urinary 8-isoprostane F-2 alpha (8-EPG) detected by ELISA. Pre- and post-intervention plasma levels of selected carotenoids were determined by HPLC. Subjects were free living and consumed a completely defined recipe-based diet that increased their average daily consumption of vegetables and fruit from 5.8 servings at baseline to 12.0 servings throughout the intervention. Overall, the level of 8-OBdG in DNA isolated from lymphocytes and in urine and the level of 8-EPG in urine were reduced by the intervention, whereas urine concentrations of MDA were minimally affected. The reduction in lymphocyte 8-OHdG was greater in magnitude (32 versus 5%) in individuals with lower average preintervention levels of plasma a-carotene (56 ng/ml) than in individuals with higher average pre-intervention plasma levels of alpha-carotene (148 ng/ml), The results of this study indicate that consumption of a diet that significantly increased vegetable and fruit intake from a diverse number of botanical families resulted in significant reductions in markers of oxidative cellular damage to DNA and lipids.
引用
收藏
页码:2261 / 2266
页数:6
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