A nucleic acid sequence-based amplification method to detect Salmonella enterica serotype enteritidis strain PT4 in liquid whole egg

被引：17

作者：

Cook, N ^{[1
]}

Ellison, J ^{[1
]}

Kurdziel, AS ^{[1
]}

Simpkins, S ^{[1
]}

Hays, JP ^{[1
]}

机构：

[1] Cent Sci Lab, York YO41 1LZ, N Yorkshire, England

来源：

JOURNAL OF FOOD PROTECTION | 2002年 / 65卷 / 07期

关键词：

D O I：

10.4315/0362-028X-65.7.1177

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Nucleic acid sequence-based amplification (NASBA) was applied to the detection of Salmonella enterica cells in liquid whole egg. Samples (25 g) of liquid whole egg inoculated with Salmonella cells were enriched for 16 h in buffered peptone water, and the NASBA procedure was effective in detecting the presence of Salmonella in samples inoculated with 10 to 100 CFU prior to enrichment.

引用

页码：1177 / 1178

页数：2

共 11 条

[1]

*BRIT STAND PUBL L, 1998, 128241998 BS EN BRIT

[2] RAPID DETECTION OF SALMONELLA-ENTERITIDIS IN POOLED LIQUID EGG SAMPLES USING A MAGNETIC BEAD-ELISA SYSTEM [J].