A long-term study on the prevalence of shiga toxin-producing Escherichia coli (STEC) on four German cattle farms

被引:80
作者
Geue, L
Segura-Alvarez, M
Conraths, FJ
Kuczius, T
Bockemühl, J
Karch, H
Gallien, P
机构
[1] Inst Epidemiol Diagnost, Bundesforsch Anstalt Viruskrankheiten, D-16868 Wusterhausen, Germany
[2] Bundesinst Gesundheitl Verbraucherschutz & Vet Me, D-06846 Dessau, Germany
[3] Natl Referenzzentrum Enteritiserreger, Hyg Inst Hamburg, D-20539 Hamburg, Germany
[4] Univ Wurzburg, Inst Hyg Mikrobiol, D-97080 Wurzburg, Germany
关键词
D O I
10.1017/S0950268802007288
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
The occurrence of Shiga toxin-producing Escherichia coli (STEC) was studied on four cattle farms. STEC were detected in 29-82% of the cattle. STEC with additional EHEC markers were detected on all farms. The occurrence of the complete virulence marker pattern (stx1 and/or stx2, eae, EHEChlyA katP, espP) was correlated with the presence of known STEC serotypes. STEC O26:H11 and O165:H25 with the complete pattern of virulence markers were the most prevalent. STEC O157 (H7/H-) STEC O103:H2 and STEC O145:H- were found sporadically. Five clonal subgroups of the STEC O26:H11 isolates were identified by pulsed-field gel electrophoresis. STEC O26:H11 were present in three groups of cattle. This serotype was detected in a single group over the entire fattening period. Most STEC O26:H11 with the complete pattern of potential virulence markers were found in clinically healthy cattle. These animals may represent a risk factor for farmers and consumers.
引用
收藏
页码:173 / 185
页数:13
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