End-group effects on the properties of PEG-co-PGA hydrogels

被引:52
作者
Bencherif, Sidi A. [1 ]
Srinivasan, Abiraman [2 ,4 ]
Sheehan, Jeffrey A. [3 ]
Walker, Lynn M. [3 ]
Gayathri, Chakicherla [1 ]
Gil, Roberto [1 ]
Hollinger, Jeffrey O. [2 ,4 ]
Matyjaszewski, Krzysztof [1 ]
Washburn, Newell R. [1 ,4 ]
机构
[1] Carnegie Mellon Univ, Dept Chem, Pittsburgh, PA 15213 USA
[2] Carnegie Mellon Univ, Bone Tissue Engn Ctr, Pittsburgh, PA 15213 USA
[3] Carnegie Mellon Univ, Dept Chem Engn, Pittsburgh, PA 15213 USA
[4] Carnegie Mellon Univ, Dept Biomed Engn, Pittsburgh, PA 15213 USA
关键词
PEG-co-PGA; End-group chemistry; Micelle; Biodegradable; Hydrogel properties; ACID-BASED HYDROGELS; TISSUE; BIOMATERIALS; NETWORKS;
D O I
10.1016/j.actbio.2009.02.030
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
A series of resorbable poly(ethylene glycol)-co-poly(glycolic acid) (PEG-co-PGA, 4KG5) macromonomers have been synthesized with the chemistries from three different photopolymerizable end-groups (acrylates, methacrylates and urethane methacrylates). The aim of the study is to examine the effects of the chemistry of the cross-linker group on the properties of photocross-linked hydrogels. 4KG5 hydrogels were prepared by photopolymerization with high vinyl group conversion as confirmed by H-1 nuclear magnetic resonance spectrometry using a 1D diffusion-ordered spectrometry pulse sequence. Our study reveals that the nature of end-groups in a moderately amphiphilic polymer can adjust the distribution and size of the micellar configuration in water, leading to changes in the macroscopic structure of hydrogels. By varying the chemistry of the cross-linker group (diacrylates (DA), dimethacrylates (DM) and urethane dimethacrylates (UDM)), we determined that the hydrophobicity of a single core polymer consisting of poly(glycolic acid) could be fine-tuned, leading to significant variations in the mechanical, swelling and degradation properties of the gels. In addition, the effects of cross-linker chemistry on cytotoxicity and proliferation were examined. Cytotoxicity assays showed that the three types of hydrogels (4KG5 DA, DM and UDM) were biocompatible and the introduction of RGD ligand enhanced cell adhesion. However, differences in gel properties and stability differentially affected the spreading and proliferation of myoblast C2C12 cells. (C) 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:1872 / 1883
页数:12
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