Identification of murine T cells reactive with the bacterial superantigen Yersinia pseudotuberculosis-derived mitogen (YPM) and factors involved in YPM-induced toxicity in mice

被引：22

作者：

MiyoshiAkiyama, T ^{[1
]}

Fujimaki, W ^{[1
]}

Yan, XJ ^{[1
]}

Yagi, J ^{[1
]}

Imanishi, K ^{[1
]}

Kato, H ^{[1
]}

Tomonari, K ^{[1
]}

Uchiyama, T ^{[1
]}

机构：

[1] FUKUSHIMA MED COLL,FUKUI 91011,JAPAN

来源：

MICROBIOLOGY AND IMMUNOLOGY | 1997年 / 41卷 / 04期

关键词：

murine T cell; superantigen; Yersinia pseudotuberculosis; YPM;

D O I：

10.1111/j.1348-0421.1997.tb01211.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We previously reported that Yersinia pseudotuberculosis-derived mitogen (YPM) acts as a superantigen to human T cells. In this study, we assessed the superantigenicity and toxicity of YPM using murine experimental models. YPM activated T cells to produce interleukin-2 in a major histocompatibility complex class II molecule-dependent manner, The T-cell blasts induced by YPM expressed T-cell receptor (TCR) beta-chain variable region (V beta)7, V beta 8.1, V beta 8.2 and V beta 8.3. The injection of YPM into mice pre-sensitized with D-galactosamine induced lethal shock. This shock was blocked by the injection of monoclonal antibodies (mAbs) to CD4, TCR V beta 7 plus V beta 8, tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma), but not by injection to CD8 or unrelated V beta. These results indicate that YPM-induced shock requires the presence of CD4(+) T cells bearing TCR V beta 7 and V beta 8, and that endogenous TNF-alpha and IFN-gamma mediate the lethal effects.

引用

页码：345 / 352

页数：8

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