EdU, a new thymidine analogue for labelling proliferating cells in the nervous system

被引：217

作者：

Chehrehasa, Fatemah ^{[1
]}

Meedeniya, Adrian C. B. ^{[1
]}

Dwyer, Patrick ^{[1
]}

Abrahamsen, Greger ^{[1
]}

Mackay-Sim, Alan ^{[1
]}

机构：

[1] Griffith Univ, Natl Ctr Adult Stem Cell Res, Eskitis Inst Cellular & Mol Therapies, Brisbane, Qld 4111, Australia

来源：

JOURNAL OF NEUROSCIENCE METHODS | 2009年 / 177卷 / 01期

关键词：

Immunofluorescence; Cell proliferation; Neurogenesis; Neural precursor; Cell differentiation; Thymidine analogue; Cell labelling; Brain; MONOCLONAL-ANTIBODY; CYCLE ANALYSIS; IN-VIVO; DNA; IMMUNOHISTOCHEMISTRY; NEUROGENESIS; LIMITATIONS; BRAIN;

D O I：

10.1016/j.jneumeth.2008.10.006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Labelling and identifying proliferating cells is central to understanding neurogenesis and neural lineages in vivo and in vitro. We present here a novel thymidine analogue, ethynyl deoxyuridine (EdU) for labelling dividing cells, detected with a fluorescent azide which forms a covalent bond via the "click" chemistry reaction (the Huisgen 1,3-dipolar cycloaddition reaction of an organic azide to a terminal acetylene). Unlike the commonly used BrdU, EdU detection requires no heat or acid treatment. It is quick and easy and compatible with multiple probes for fluorescence immunochemistry, facilitating the characterisation of proliferating cells at high resolution. (C) 2008 Elsevier B.V. All rights reserved.

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页码：122 / 130

页数：9

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