Biological activity of functionalized SiO2 thin films prepared by sol-gel method

被引:10
作者
Collino, R
Therasse, J
Chaput, F
Boilot, JP
Levy, Y
机构
[1] ECOLE POLYTECH, PHYS MAT CONDENSEE LAB, GRP CHIM SOLIDE, CNRS URA D1254, F-91128 PALAISEAU, FRANCE
[2] INST OPT THEORET & APPL, LAB OPT NON LINEAIRE, F-91403 ORSAY, FRANCE
关键词
sol-gel; functionalized films; grafting of antibodies; biological activity;
D O I
10.1007/BF00401887
中图分类号
TQ174 [陶瓷工业]; TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
In this work we investigated the biological properties of sol-gel films in aqueous medium. Functionalized silica films were prepared by the sol-gel process, from organically modified silicon alkoxides with amino or thiol groups. Covalent binding of proteins with different orientations according to the hydrophilic or hydrophobic character of the surface was studied. This binding occurred via a covalent coupling agent providing a very stable linkage. No denaturation was detected and a good detection of the antigen was observed. Immunoassays have demonstrated the biological activity of grafted antibodies.
引用
收藏
页码:81 / 85
页数:5
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