Chromomycin A3 staining as a useful tool for evaluation of male fertility

被引：83

作者：

Iranpour, FG ^{[1
]}

Nasr-Esfahani, MH ^{[1
]}

Valojerdi, MR ^{[1
]}

Al-Taraihi, TMT ^{[1
]}

机构：

[1] Tarbiat Modarres Univ, Sch Med, Dept Anat, Tehran, Iran

来源：

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 2000年 / 17卷 / 01期

关键词：

chromomycin A(3); fertilization; IVF; spermatozoa;

D O I：

10.1023/A:1009406231811

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Purpose: Our purpose was to investigate the association between percentage chromomycin A(3) (CMA(3)) positivity of spermatozoa with some sperm parameters and in vitro fertilization rate. Methods: Spermatozoa were collected from 139 men, washed in PBS, fixed in methanol/glacial acetic acid (3:1), and then spread on slides. CMA(3) positivity is expressed as the percentage in 200 spermatozoa. Results: Percentage of CMA(3) positivity showed not only a negative con-elation with fertilization rate but also a significant difference between fertilizing and nonfertilizing patients. Moreover percentage of CMA(3)-positive spermatozoa showed a negative correlation with count and percentage motility and a positive correlation with percentage of abnormal morphology. Percentage of CMA(3) positivity also had a positive correlation with some abnormalities of head such as amorphous and macrocephaly. Ultrastructural study showed chromatin unpackaging in high CMA(3)-positive semen samples in comparison with low CMA(3)-positive semen samples. Conclusion: There is a close relationship among fertilization rate, sperm parameters, and CMA(3) positivity and CMA(3) could be considered as a useful tool for evaluation of male fertility prior to infertility treatment.

引用

页码：60 / 66

页数：7

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