Leucyl-tRNA synthetase from the extreme thermophile Aquifex aeolicus has a heterodimeric quaternary structure

被引：11

作者：

Gouda, M ^{[1
]}

Yokogawa, T ^{[1
]}

Asahara, H ^{[1
]}

Nishikawa, K ^{[1
]}

机构：

[1] Gifu Univ, Fac Engn, Dept Biomol Sci, Gifu 5011193, Japan

来源：

FEBS LETTERS | 2002年 / 518卷 / 1-3期

关键词：

aminoacylation; leucyl-tRNA synthetase; heterodimer; quaternary structure; Aquifex aeolicus;

D O I：

10.1016/S0014-5793(02)02675-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Class I aminoacyl-tRNA synthetases have been thought to be single polypeptide enzymes. However, the complete genome sequence of a hyper thermophile Aquifex aeolicus suggests that the gene for leucyl-tRNA synthetases (LeuRS) is probably split into two pieces (leuS and leuS). In this research, each gene was separately cloned and overexpressed in Escherichia coli and the protein products were examined for LeuRS activity. Leucylation activity was detected only when both gene products coexisted. Gel filtration analysis showed that the active form of A. aeolicus LeuRS has a heterodimeric (alpha/beta type) quaternary structure that is unique among class I aminoacyl-tRNA synthetases. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：139 / 143

页数：5

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