Rod-Like Microglia Are Restricted to Eyes with Laser-Induced Ocular Hypertension but Absent from the Microglial Changes in the Contralateral Untreated Eye

被引：78

作者：

de Hoz, Rosa ^{[1
,2
]}

Gallego, Beatriz I. ^{[1
,2
]}

Ramirez, Ana I. ^{[1
,2
]}

Rojas, Blanca ^{[1
,3
]}

Salazar, Juan J. ^{[1
,2
]}

Valiente-Soriano, Francisco J. ^{[4
]}

Aviles-Trigueros, Marcelino ^{[4
]}

Villegas-Perez, Maria P. ^{[4
]}

Vidal-Sanz, Manuel ^{[4
]}

Trivino, Alberto ^{[1
,3
]}

Ramirez, Jose M. ^{[1
,3
]}

机构：

[1] Univ Complutense Madrid, Inst Invest Oftalmol Ramon Castroviejo, Madrid, Spain

[2] Univ Complutense Madrid, Fac Opt & Optometria, Madrid, Spain

[3] Univ Complutense Madrid, Fac Med, Madrid, Spain

[4] Univ Murcia, Dept Ophthalmol, Sch Med, Murcia, Spain

来源：

PLOS ONE | 2013年 / 8卷 / 12期

关键词：

ELEVATED INTRAOCULAR-PRESSURE; RETINAL GANGLION-CELLS; OPTIC-NERVE HEAD; GENE-EXPRESSION; HUMAN GLAUCOMA; RAT MODEL; INJURY; DEGENERATION; RESPONSES; ACTIVATION;

D O I：

10.1371/journal.pone.0083733

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

In the mouse model of unilateral laser-induced ocular hypertension (OHT) the microglia in both the treated and the normotensive untreated contralateral eye have morphological signs of activation and up-regulation of MHC-II expression in comparison with naive. In the brain, rod-like microglia align to less-injured neurons in an effort to limit damage. We investigate whether: i) microglial activation is secondary to laser injury or to a higher IOP and; ii) the presence of rod-like microglia is related to OHT. Three groups of mice were used: age-matched control (naive, n=15); and two lasered: limbal (OHT, n=15); and non-draining portion of the sclera (scleral, n=3). In the lasered animals, treated eyes as well as contralateral eyes were analysed. Retinal whole-mounts were immunostained with antibodies against, Iba-1, NF-200, MHC-II, CD86, CD68 and Ym1. In the scleral group (normal ocular pressure) no microglial signs of activation were found. Similarly to naive eyes, OHT-eyes and their contralateral eyes had ramified microglia in the nerve-fibre layer related to the blood vessel. However, only eyes with OHT had rod-like microglia that aligned end-to-end, coupling to form trains of multiple cells running parallel to axons in the retinal surface. Rod-like microglia were CD68+ and were related to retinal ganglion cells (RGCs) showing signs of degeneration (NF-200+ RGCs). Although MHC-II expression was up-regulated in the microglia of the NFL both in OHT-eyes and their contralateral eyes, no expression of CD86 and Ym1 was detected in ramified or in rod-like microglia. After 15 days of unilateral lasering of the limbal and the non-draining portion of the sclera, activated microglia was restricted to OHT-eyes and their contralateral eyes. However, rod-like microglia were restricted to eyes with OHT and degenerated NF-200+ RGCs and were absent from their contralateral eyes. Thus, rod-like microglia seem be related to the neurodegeneration associated with HTO.

引用

页数：19

共 83 条

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