Retention of ionizable compounds on HPLC.: Modelling retention in reversed-phase liquid chromatography as a function of pH and solvent composition with methanol-water mobile phases

被引：24

作者：

Canals, I ^{[1
]}

Portal, JA ^{[1
]}

Rosés, M ^{[1
]}

Bosch, E ^{[1
]}

机构：

[1] Univ Barcelona, Dept Quim Analit, E-08028 Barcelona, Spain

来源：

CHROMATOGRAPHIA | 2002年 / 55卷 / 9-10期

关键词：

column liquid chromatography; retention models; mobile phase pH measurement; polarity parameters; dissociation constants;

D O I：

10.1007/BF02492902

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The retention of ionizable compounds in reversed-phase liquid chromatography with methanol-water mobile phases has been related to the pH, composition and polarity of the mobile phase. The ionization of the compound is calculated from the pH measured in the mobile phase ((w)(w)pH) and the pK(a) of the compound in the same mobile phase ((s)(w)pK(a)) which can be estimated from the aqueous pK(a) ((w)(w)pK(a)) by means of general equations previously established. The retention factors are calculated from the degree of ionization and the retention factors of the neutral and ionic forms of the compounds. Two different linear models have been tested to relate the retention factor of the acid-base forms of the studied compounds to methanol-water composition or polarity.

引用

页码：565 / 571

页数：7

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