Development of a sensitive method for the determination of ganciclovir by reversed-phase high-performance liquid chromatography

被引:38
作者
Merodio, M
Campanero, MA
Mirshahi, T
Mirshahi, M
Irache, JM
机构
[1] Univ Navarra, Ctr Galenico, E-31080 Pamplona, Spain
[2] Univ Navarra Clin, Serv Farmacol Clin, Pamplona 31080, Spain
[3] Univ Paris 06, INSERM, XR86, F-75006 Paris, France
关键词
pharmaceutical analysis; ganciclovir; acyclovir; nucleoside analogues; albumin;
D O I
10.1016/S0021-9673(99)01015-8
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Ganciclovir is a nucleoside analogue widely used in the treatment of cytomegalovirus infections, which affects mainly immunocompromised patients. Recently, new pharmaceutical dosage forms based on the use of albumin nanoparticles have been developed for improving the efficacy of this drug. The aim of this study was to develop an analytical HPLC method for the determination of ganciclovir in both pharmaceuticals (i.e. albumin nanoparticles) and biological medium samples. The chromatography was performed on a reversed-phase encapped column (LiChrospher Select B C-8) with a mobile phase consisting of acetonitrile in 0.05 M ammonium acetate (pH 6.5; 2: 98, v/v). Acyclovir was used as internal standard and the detection wavelength was 254 nm. The limit of quantitation of ganciclovir was 50 ng/ml and the average recoveries over a concentration range of 0.05-10 mu g/ml ranged from 98 to 102%. Precision did not exceed 5%. In summary, this assay is a selective, sensitive and reproducible method for the determination of the ganciclovir in albumin nanoparticles. It can be successfully applied to the estimation of the ganciclovir uptake by cultured human corneal fibroblasts. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:159 / 167
页数:9
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