Nonpolarized cells selectively sort apical proteins from cell surface to a novel compartment, but lack apical retention mechanisms

被引:37
作者
Tuma, PL [1 ]
Nyasae, LK [1 ]
Hubbard, AL [1 ]
机构
[1] Johns Hopkins Univ, Sch Med, Dept Cell Biol, Baltimore, MD 21205 USA
关键词
D O I
10.1091/mbc.02-04-0054
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Membrane trafficking is central to establishing and maintaining epithelial cell polarity. One open question is to what extent the mechanisms regulating membrane trafficking are conserved between nonpolarized and polarized cells. To answer this question, we examined the dynamics of domain-specific plasma membrane (PM) proteins in three classes of hepatic cells: polarized and differentiated WIF-B cells, nonpolarized and differentiated Fao cells, and nonpolarized and nondifferentiated Clone 9 cells. In nonpolarized cells, mature apical proteins were uniformly distributed in the PM. Surprisingly, they were also in an intracellular compartment. Double labeling revealed that the compartment contained only apical proteins. By monitoring the dynamics of antibody-labeled molecules in nonpolarized cells, we further found that apical proteins rapidly recycled between the compartment and PM. In contrast, the apical PM residents in polarized cells showed neither internalization nor return to the basolateral PM from which they had originally come. Cytochalasin D treatment of these polarized cells revealed that the retention mechanisms are actin dependent. We conclude from these data that both polarized and nonpolarized cells selectively sort apical proteins from the PM and transport them to specific, but different cellular locations. We propose that the intracellular recycling compartment in nonpolarized cells is an intermediate in apical surface formation.
引用
收藏
页码:3400 / 3415
页数:16
相关论文
共 55 条
[1]   Endocytic traffic in polarized epithelial cells: Role of the actin and microtubule cytoskeleton [J].
Apodaca, G .
TRAFFIC, 2001, 2 (03) :149-159
[2]   Myosins: matching functions with motors [J].
Baker, JP ;
Titus, MA .
CURRENT OPINION IN CELL BIOLOGY, 1998, 10 (01) :80-86
[3]   NEWLY SYNTHESIZED HEPATOCYTE PLASMA-MEMBRANE PROTEINS ARE TRANSPORTED IN TRANSCYTOTIC VESICLES IN THE BILE-DUCT LIGATED RAT [J].
BARR, VA ;
HUBBARD, AL .
GASTROENTEROLOGY, 1993, 105 (02) :554-571
[4]   PRESERVATION OF HEPATOCYTE PLASMA-MEMBRANE DOMAINS DURING CELL-DIVISION INSITU IN REGENERATING RAT-LIVER [J].
BARTLES, JR ;
HUBBARD, AL .
DEVELOPMENTAL BIOLOGY, 1986, 118 (01) :286-295
[5]   PLASMA-MEMBRANE PROTEIN SORTING IN EPITHELIAL-CELLS - DO SECRETORY PATHWAYS HOLD THE KEY [J].
BARTLES, JR ;
HUBBARD, AL .
TRENDS IN BIOCHEMICAL SCIENCES, 1988, 13 (05) :181-184
[6]   BIOGENESIS OF THE RAT HEPATOCYTE PLASMA-MEMBRANE INVIVO - COMPARISON OF THE PATHWAYS TAKEN BY APICAL AND BASOLATERAL PROTEINS USING SUBCELLULAR FRACTIONATION [J].
BARTLES, JR ;
FERACCI, HM ;
STIEGER, B ;
HUBBARD, AL .
JOURNAL OF CELL BIOLOGY, 1987, 105 (03) :1241-1251
[7]   Absence of direct delivery for single transmembrane apical proteins or their "secretory" forms in polarized hepatic cells [J].
Bastaki, M ;
Braiterman, LT ;
Johns, DC ;
Chen, YH ;
Hubbard, AL .
MOLECULAR BIOLOGY OF THE CELL, 2002, 13 (01) :225-237
[8]   ERM-merlin and EBP50 protein families in plasma membrane organization and function [J].
Bretscher, A ;
Chambers, D ;
Nguyen, R ;
Reczek, D .
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 2000, 16 :113-+
[9]   Truncated brush border myosin I affects membrane traffic in polarized epithelial cells [J].
Durrbach, A ;
Raposo, G ;
Tenza, D ;
Louvard, D ;
Coudrier, E .
TRAFFIC, 2000, 1 (05) :411-424
[10]   Brush border myosin-I truncated in the motor domain impairs the distribution and the function of endocytic compartments in an hepatoma cell line [J].
Durrbach, A ;
Collins, K ;
Matsudaira, P ;
Louvard, D ;
Coudrier, E .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (14) :7053-7058