Isolation and identification of nondestructive desulfurization bacterium

被引:11
作者
Gou, ZX [1 ]
Liu, HZ [1 ]
Luo, MF [1 ]
Li, S [1 ]
Xing, JM [1 ]
Chen, JY [1 ]
机构
[1] Chinese Acad Sci, State Key Lab Biochem Engn, Young Sci Lab Separat Sci & Engn, Beijing 100080, Peoples R China
来源
SCIENCE IN CHINA SERIES B-CHEMISTRY | 2002年 / 45卷 / 05期
关键词
dibenzothiophene; isolation; identification; biodesulfurization; Rhodococcus erythropolis;
D O I
10.1360/02yb9069
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A nondestructive desulfurization microorganism has been isolated. The metabolism product analyses show that the strain can be a kind of biocatalyst to oxidize dibenzothlophene (DBT) into 2-hydroxydiphenyl (HBP), therefore the sulfur in DBT is removed selectively. The 16SrRNA information, cell wall analysis, physical, biochemical properties and morphological properties suggest that the isolated strain is Rhodococcus etythropolis. The strain can grow in the basal salts medium (BSM) that DBT concentration is no more than 10 mmol/L, and the optimal DBT concentration for growth is 1 mmol/L, however, the optimal DBT concentration for desulfurization is 0.5 mmol/L. The further research shows that the strain can also desulfur some other organosulfur-containing compounds such as thianaphthene, phenyl sulfide and 4,6-dimethyldibenzothiophene (4,6-DMDBT).
引用
收藏
页码:521 / 531
页数:11
相关论文
共 19 条
[1]  
CHRISTOPHER O, 1997, MICROBIOLOGY, V143, P2961
[2]   IDENTIFICATION AND CLONING OF GENES INVOLVED IN SPECIFIC DESULFURIZATION OF DIBENZOTHIOPHENE BY RHODOCOCCUS SP STRAIN IGTS8 [J].
DENOME, SA ;
OLSON, ES ;
YOUNG, KD .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (09) :2837-2843
[3]  
JIANG CY, 2001, THESIS CHINESE ACAD
[4]  
KEVIN AG, 1996, NAT BIOTECHNOL, V14, P1705
[5]  
Kilbane IIJJ, 1994, US Patent, Patent No. [5,358,869, 5358869]
[6]   DESULFURIZATION OF COAL - THE MICROBIAL SOLUTION [J].
KILBANE, JJ .
TRENDS IN BIOTECHNOLOGY, 1989, 7 (04) :97-101
[7]  
KILBANE JJ, 1992, Patent No. 5132219
[8]  
KILBANE JJ, 1994, Patent No. 5344778
[9]  
KILBANE JJ, 1992, Patent No. 5104801
[10]  
KODAMA K, 1973, AGR BIOL CHEM TOKYO, V37, P45, DOI 10.1080/00021369.1973.10860640