Analysis of immunoglobulin VH and TCR Cβ polymorphisms in a large family with thyroid autoimmune disorder

被引：11

作者：

Fakhfakh, F

Maalej, A

Makni, H

Abid, M

Jouida, J

Zouali, M

Ayadi, H ^{[1
]}

机构：

[1] Fac Med, Lab Immunol & Biol Mol, TN-3018 Sfax, Tunisia

[2] Serv Endocrinol EPS Hedi Chaker, Sfax, Tunisia

[3] Dispensaire Bir El Hfai, Sidi Bouzid, Tunisia

[4] Inst Pasteur, Dept Immunol, Paris, France

来源：

EXPERIMENTAL AND CLINICAL IMMUNOGENETICS | 1999年 / 16卷 / 04期

关键词：

thyroid autoimmune disorder; immunoglobulin genes; T-cell receptor C beta genes; restriction fragment length polymorphism analysis;

D O I：

10.1159/000019110

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to investigate the association of TCR C beta and immunoglobulin (Ig) VH polymorphisms with thyroid autoimmune diseases (TAD), we analyzed restriction-endonuclease-generated polymorphisms using T-cell receptor (TCR) C beta and VH gene-family-specific probes. We tested genomic DNAs of patients isolated from a large family affected with Graves' disease and Hashimoto's thyroiditis as well as the genomic DNA of unrelated Tunisian controls. Hybridization of Bg/II-digested DNA with a TCR C beta probe revealed two alleles of 9.2 and 10 kb. These C beta polymorphisms have already been found in the Caucasian population. However, there was no abnormal distribution of this polymorphism in patients with TAD, compared to related healthy individuals and to unrelated Tunisian controls. Besides, there was a low VH polymorphism in members of the family affected with TAD. Analysis of the Ig gene families revealed no restriction site polymorphism pattern specific for TAD. Copyright (C) 1999 S. Karger AG, Basel.

引用

页码：185 / 191

页数：7

共 31 条

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