Calcium-independent inhibition of glucose transport in PC-12 and L6 cells by calcium channel antagonists

被引:19
作者
Ardizzone, TD
Lu, XH
Dwyer, DS
机构
[1] Louisiana State Univ, Hlth Sci Ctr, Dept Pharmacol, Shreveport, LA 71130 USA
[2] Louisiana State Univ, Hlth Sci Ctr, Dept Psychiat, Shreveport, LA 71130 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 2002年 / 283卷 / 02期
关键词
glucose transporter; hyperglycemia; nimodipine; verapamil;
D O I
10.1152/ajpcell.00451.2001
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The goal of these studies was to determine whether different calcium channel antagonists affect glucose transport in a neuronal cell line. Rat pheochromocytoma (PC-12) cells were treated with L-, T-, and N-type calcium channel antagonists before measurement of accumulation of 2-[H-3] deoxyglucose (2- H-3] DG). The L-type channel antagonists nimodipine, nifedipine, verapamil, and diltiazem all inhibited glucose transport in a dose-dependent manner (2- 150 muM) with nimodipine being the most potent and diltiazem only moderately inhibiting transport. T- and N-type channel antagonists had no effect on transport. The L- type channel agonist l-BAY K 8644 also inhibited uptake of 2-[H-3] DG. The ability of these drugs to inhibit glucose transport was significantly diminished by the presence of unlabeled 2- DG in the uptake medium. Some experiments were performed in the presence of EDTA (4 mM) or in uptake buffer without calcium. The absence of calcium in the uptake medium had no effect on inhibition of glucose transport by nimodipine or verapamil. To examine the effects of these drugs on a cell model of a peripheral tissue, we studied rat L6 muscle cells. The drugs inhibited glucose transport in L6 myoblasts in a dose-dependent manner that was independent of calcium in the uptake medium. These studies suggest that the calcium channel antagonists inhibit glucose transport in cells through mechanisms other than the antagonism of calcium channels, perhaps by acting directly on glucose transporters.
引用
收藏
页码:C579 / C586
页数:8
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