Pemphigus vulgaris autoantibodies induce apoptosis in HaCaT keratinocytes

被引：55

作者：

Pelacho, B

Natal, C

España, A

Sánchez-Carpintero, I

Iraburu, MJ

López-Zabalza, MJ

机构：

[1] Univ Navarra, Dept Biochem, E-31080 Pamplona, Spain

[2] Univ Navarra, Clin Univ, Dept Dermatol, E-31080 Pamplona, Spain

来源：

FEBS LETTERS | 2004年 / 566卷 / 1-3期

关键词：

pemphigus vulgaris; desmoglein; acantholysis; apoptosis; human keratinocyte; IgG autoantibody;

D O I：

10.1016/j.febslet.2004.03.107

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pemphigus vulgaris (PV) is an autoimmune disease characterized by binding of IgG autoantibodies to epidermal keratinocyte desmosomes. IgG autoantibodies obtained from a patient with mucocutaneous PV reacted with plakoglobin (Plkg) in addition to desmoglein-3 (Dsg3) and Dsg1. Immunofluorescence analysis confirmed that IgG autoantibodies, unlike antibodies from a healthy volunteer, caused disruption of cell-cell contacts in HaCaT keratinocytes. Moreover, apoptosis was enhanced in cells treated with autoantibodies compared to those treated with normal antibodies. The apoptotic process induced by IgG autoantibodies was characterized by caspase-3 activation, Bcl-2 depletion and Bax expression. The present report demonstrates that PV IgG autoantibodies promote apoptosis in HaCaT keratinocytes. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：6 / 10

页数：5

共 38 条

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