Quantification of intra- and extra-cellular thermophilic lipase/esterase production by Thermus sp.

被引：29

作者：

Domínguez, A

Sanromán, A

Fuciños, P

Rúa, ML

Pastrana, L

Longo, MA ^{[1
]}

机构：

[1] Univ Vigo, Dept Chem Engn, Vigo 36200, Spain

[2] Univ Vigo, Dept Biochem Genet & Immunol, Vigo 36200, Spain

来源：

BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 09期

关键词：

esterase; extremophiles; lipase; thermophilic; Thermus;

D O I：

10.1023/B:BILE.0000024092.27943.75

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Four Thermus strains produced lipolytic activity when grown in liquid medium for 30 h at 70degreesC. The highest total lipase/esterase activity (57 U l(-1)) was in Thermus aquaticus YT-1, followed by Thermus thermophilus HB27 and HB8 (33 and 25 U l(-1), respectively), and finally by Thermus sp. (16 U l(-1)). Extra-cellular activity was detected in T. aquaticus YT-1 and T. thermophilus HB27 (33 and 17 U l(-1)). All enzymes were stable at 80. C over 30 min, and their activity towards fatty acid esters increased as substrate chain-length diminished (i.e. hydrolysis rate was up to 6-fold higher on p-nitrophenyl caproate than on laurate).

引用

页码：705 / 708

页数：4

共 14 条

[1] Response of the thermophile Thermus sp RQ-1 to hyperbaric air in batch and fed-batch cultivation [J].