Analysis of vervain flavonoids by HPLC/diode array detector method. Its application to quality control

被引：54

作者：

Valentao, P

Andrade, PB

Areias, F

Ferreres, F

Seabra, RM

机构：

[1] Univ Porto, Fac Farm, Lab Farmacognosia, CEQUP, P-4050 Porto, Portugal

[2] CSIC, CEBAS, Dept Ciencia & Tecnol Alimentos, Lab Fitoquim, E-30080 Murcia, Spain

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1999年 / 47卷 / 11期

关键词：

vervain (Lippia citriodora); HPLC/DAD detector; flavonoids; chemical fingerprint; quality control;

D O I：

10.1021/jf990444i

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A reversed-phase HPLC procedure is proposed for the determination of seven flavonoids (luteolin, nepetin, hispidulin, jaceosidin, cirsimaritin, cirsilineol, and eupatorin) in vervain samples. A simple extractive technique was developed, involving only extraction with ethyl ether, which allowed the elimination of phenolic acids, yielding a sufficiently purified flavonoid fraction. All of the analyzed vervain samples showed a common flavonoid pattern, in which hispidulin and jaceosidin were the major compounds and nepetin was the minor compound.

引用

页码：4579 / 4582

页数：4