Structural features and anticancer activity in vitro of fucoidan derivatives from brown alga Saccharina cichorioides

被引：54

作者：

Anastyuka, Stanislav D. ^{[1
]}

Shevchenko, Natalia M. ^{[1
]}

Usoltseva , Roza V. ^{[1
]}

Silchenko, Artyom S. ^{[1
]}

Zadorozhny, Pavel A. ^{[2
]}

Dmitrenok, Pavel S. ^{[1
]}

Ermakova, Svetlana P. ^{[1
]}

机构：

[1] Russian Acad Sci, Far Eastern Branch, GB Elyakov Pacific Inst Bioorgan Chem, 100 Let Vladivostoku Prosp,159-2, Vladivostok 690022, Russia

[2] Russian Acad Sci, Far Eastern Branch, Inst Chem, 100 Let Vladivostoku Prosp,159D, Vladivostok 690022, Russia

来源：

CARBOHYDRATE POLYMERS | 2017年 / 157卷

基金：

俄罗斯科学基金会;

关键词：

Fucoidan derivatives; Structure; Mass spectrometry; NMR; C-PAGE; Anticancer activity; MASS-SPECTROMETRY; POLYSACCHARIDES; HYDROLYSIS; ESIMS;

D O I：

10.1016/j.carbpol.2016.11.031

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

A fucoidan ScF from brown alga Saccharina cichorioides was extracted, purified and partially depolymerized by autohydrolysis at 37 degrees C for 24, 48 and 72 h. Supernatant (SN) and pellet (PL) fractions were obtained by ethanol precipitation of each sample. Unlike spectral data of ScF, NMR of PL derivatives clearly suggested the structure: 1,3-linked alpha-L-Fucp-4-OSO3- repeating unit. Molecular weights (MWs) of PL fractions were 30, 26 and 18 kDa for 24, 48 and 72 h of autohydrolyis, respectively. MALDI-TOFMS, size-exclusion HPLC and carbohydrate polyacrylamide-gel electrophoresis (C-PAGE) indicated a similarity of SN mixtures. They consisted mainly of a polysaccharide part (MW 6 kDa, C-PAGE data) with a structure similar to PL components (NMR data) and monosaccharides alpha-L-Fucp-4-OSO3-, alpha-L-Fucp-2,4-di-OSO3-. PL fractions exhibited almost identical antiproliferative activity in vitro as native fucoidan, while an SN sample for 72 h of autohydrolysis was slightly more active against colony formation of colorectal carcinoma cells HT-29. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：1503 / 1510

页数：8

共 30 条

[1]

Important Determinants for Fucoidan Bioactivity: A Critical Review of Structure-Function Relations and Extraction Methods for Fucose-Containing Sulfated Polysaccharides from Brown Seaweeds [J].