Simultaneous determination of guanine and adenine in DNA using an electrochemically pretreated glassy carbon electrode

被引：179

作者：

Wang, HS ^{[1
]}

Ju, HX ^{[1
]}

Chen, HY ^{[1
]}

机构：

[1] Nanjing Univ, Inst Sci Anal, Dept Chem, State Key Lab Coordinat Chem, Nanjing 210093, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2002年 / 461卷 / 02期

基金：

中国国家自然科学基金;

关键词：

guanine; adenine; DNA; adsorptive stripping voltammetry; glassy carbon electrode; electrochemical pretreatment;

D O I：

10.1016/S0003-2670(02)00297-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple and reliable method based on adsorptive stripping at an electrochemically pretreated glassy carbon electrode (GCE) was proposed for simultaneous or individual determination of guanine and adenine in DNA. The detection sensitivity of guanine and adenine was improved greatly by activating the GCE electrochemically. After accumulation on pretreated GCE at open circuit for 5 min or at the potential of +0.3 V for 120 s, guanine and adenine produced well-defined oxidation peaks at about +0.8 and +1.1 V, respectively in pH 5 phosphate buffer. The detection limit for individual measurement of guanine and adenine was 4.5 ng ml(-1) (3 x 10(-8) mol l(-1)) and 4 ng ml(-1) (3 x 10(-8) mol l(-1)), respectively. Acid-denatured DNA showed two oxidation peaks corresponding to guanine and adenine residues in the same buffer. The proposed method can be used to estimate the guanine and adenine contents in DNA with good selectivity in a linear range of 0.25-5 mug ml(-1). (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：243 / 250

页数：8

共 49 条

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