Statistical optimization of thermo-tolerant xylanase activity from Amazon isolated Bacillus circulans on solid-state cultivation

被引：38

作者：

Heck, Julio Xandro ^{[1
]}

Flores, Simone Hickmann ^{[1
]}

Hertz, Plinho Francisco ^{[1
]}

Zachia Ayub, Marco Antonio ^{[1
]}

机构：

[1] Fed Univ Rio Grande Sul State, Inst Food Sci & Technol, BR-91501970 Porto Alegre, RS, Brazil

来源：

BIORESOURCE TECHNOLOGY | 2006年 / 97卷 / 15期

关键词：

xylanase; enzyme technology; optimization; bioconversion; solid-state cultivation; bioprocess design; biodiversity;

D O I：

10.1016/j.biortech.2005.08.013

中图分类号：

S2 [农业工程];

学科分类号：

0828 [农业工程];

摘要：

A 2(2) factorial design was performed to find the best conditions of pH and temperature for xylanolytic activity of Bacillus circulans BL53 isolated from the Amazon environment. Solid-state cultivation was carried out on an inexpensive, abundant agro-industrial soybean residue. The central composite design (CCD) used for the analysis of treatment combinations showed that a second-order polynomial regression model was in good agreement with experimental results, with R-2 = 0.9369 (P < 0.05). The maximum activity was obtained at a high temperature (80 degrees C) and over a large pH range (4.0-7.0). Enzymatic activity was maintained in heated extracts up to 50 degrees C, suggesting that the xylanases of B. circulans BL53 are thermo-tolerant biocatalysts, being of interest for industrial processes. The crude enzyme extract hydrolyzed rice straw, sugar cane bagasse and soybean fiber and its activity was stimulated by Co2+, Fe3+, and beta-mercaptoethanol but inhibited by Mn2+, Cu2+, Ca2+, Zn2+, Ba2+, Mg2+ and by EDTA. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：1902 / 1906

页数：5

共 23 条

[1]

Response surface methodological approach to optimize the nutritional parameters for neomycin production by Streptomyces marinensis under solid-state fermentation [J].