Expressed sequence tags from Madagascar periwinkle (Catharanthus roseus)

被引：42

作者：

Murata, Jun

Bienzle, Dorothee

Brandle, Jim E.

Sensen, Christoph W.

De Luca, Vincenzo

机构：

[1] Brock Univ, Dept Biol Sci, St Catharines, ON L2S 3A1, Canada

[2] Univ Guelph, Ontario Vet Coll, Guelph, ON N1G 2W1, Canada

[3] Agr & Agri Food Canada, So Crop Protect & Food Res Ctr, London, ON N5V 4T3, Canada

[4] Univ Calgary, Fac Med, Dept Biochem & Mol Biol, Calgary, AB T2N 4N1, Canada

来源：

FEBS LETTERS | 2006年 / 580卷 / 18期

基金：

加拿大自然科学与工程研究理事会;

关键词：

expressed sequence tags; secondary metabolism; Catharanthus roseus;

D O I：

10.1016/j.febslet.2006.07.020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Madagascar periwinkle (Catharanthus roseus) is well known to produce the chemotherapeutic anticancer agents, vinblastine and vincristine. In spite of its importance, no expressed sequence tag (EST) analysis of this plant has been reported. Two cDNA libraries were generated from RNA isolated from the base part of young leaves and from root tips to select 9824 random clones for unidirectional sequencing, to yield 3327 related sequences and 1696 singletons by cluster analysis. Putative functions of 3663 clones were assigned, from 5023 non-redundant ESTs to establish a resource for transcriptome analysis and gene discovery in this medicinal plant. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

收藏

页码：4501 / 4507

页数：7

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