GTP cyclohydrolase I mRNA induction and tetrahydrobiopterin synthesis in human endothelial cells

被引:29
作者
Hattori, Y [1 ]
Nakanishi, N [1 ]
Kasai, K [1 ]
Shimoda, SI [1 ]
机构
[1] MEIKAI UNIV,SCH DENT,DEPT BIOCHEM,SAKADO,SAITAMA 35002,JAPAN
来源
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1997年 / 1358卷 / 01期
关键词
GTP cyclohydrolase I; tetrahydrobiopterin; endothelial cell; cytokine;
D O I
10.1016/S0167-4889(97)00052-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The key role of tetrahydrobiopterin (BH4) in the synthesis of nitric oxide by human umbilical vein endothelial cells (HUVEC) has been demonstrated. We characterized the induction of BH4 synthesis in a cell line (ECV) derived from HUVEC and primary HUVEC. A significant induction of guanosine triphosphate cyclohydrolase I (GTPCH) mRNA was observed in response to TNF, IL-1 beta, and IFN gamma in ECV and HUVEC. The induction of GTPCH mRNA was abolished by actinomycin D. The cytokines led to an increased accumulation of BH4 in ECV. This effect was prevented by 2,4-diamino-6-hydroxypyrimidine, a selective inhibitor of GTPCH, as well as by actinomycin D and by cycloheximide. Results provide evidence for an increase in GTPCH activity and in BH4 levels in response to immunostimulants in human endothelial cells. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:61 / 66
页数:6
相关论文
共 28 条
[1]   NG-METHYLARGININE, AN INHIBITOR OF ENDOTHELIUM-DERIVED NITRIC-OXIDE SYNTHESIS, IS A POTENT PRESSOR AGENT IN THE GUINEA-PIG - DOES NITRIC-OXIDE REGULATE BLOOD-PRESSURE INVIVO [J].
AISAKA, K ;
GROSS, SS ;
GRIFFITH, OW ;
LEVI, R .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 160 (02) :881-886
[2]   THE COMPLETE SEQUENCE OF A FULL LENGTH CDNA FOR HUMAN-LIVER GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE - EVIDENCE FOR MULTIPLE MESSENGER-RNA SPECIES [J].
ARCARI, P ;
MARTINELLI, R ;
SALVATORE, F .
NUCLEIC ACIDS RESEARCH, 1984, 12 (23) :9179-9189
[3]   SHEAR-STRESS INDUCED RELEASE OF NITRIC-OXIDE FROM ENDOTHELIAL-CELLS GROWN ON BEADS [J].
BUGA, GM ;
GOLD, ME ;
FUKUTO, JM ;
IGNARRO, LJ .
HYPERTENSION, 1991, 17 (02) :187-193
[4]   Inhibition of tetrahydrobiopterin synthesis reduces in vivo nitric oxide production in experimental endotoxic shock [J].
Bune, AJ ;
Brand, MP ;
Heales, SJR ;
Shergill, JK ;
Cammack, R ;
Cook, HT .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1996, 220 (01) :13-19
[5]   IDENTIFICATION OF A COMMON NUCLEOTIDE-SEQUENCE IN THE 3'-UNTRANSLATED REGION OF MESSENGER-RNA MOLECULES SPECIFYING INFLAMMATORY MEDIATORS [J].
CAPUT, D ;
BEUTLER, B ;
HARTOG, K ;
THAYER, R ;
BROWNSHIMER, S ;
CERAMI, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (06) :1670-1674
[6]   GAMMA-INTERFERON ENHANCES MACROPHAGE TRANSCRIPTION OF THE TUMOR-NECROSIS-FACTOR CACHECTIN, INTERLEUKIN-1, AND UROKINASE GENES, WHICH ARE CONTROLLED BY SHORT-LIVED REPRESSORS [J].
COLLART, MA ;
BELIN, D ;
VASSALLI, JD ;
DEKOSSODO, S ;
VASSALLI, P .
JOURNAL OF EXPERIMENTAL MEDICINE, 1986, 164 (06) :2113-2118
[7]   TETRAHYDROBIOPTERIN AND DYSFUNCTION OF ENDOTHELIAL NITRIC-OXIDE SYNTHASE IN CORONARY-ARTERIES [J].
COSENTINO, F ;
KATUSIC, ZS .
CIRCULATION, 1995, 91 (01) :139-144
[8]   ANALYSIS OF REDUCED FORMS OF BIOPTERIN IN BIOLOGICAL TISSUES AND FLUIDS [J].
FUKUSHIMA, T ;
NIXON, JC .
ANALYTICAL BIOCHEMISTRY, 1980, 102 (01) :176-188
[9]  
Griffith O.W., 1996, METHODS NITRIC OXIDE, P187
[10]  
GROSS SS, 1992, J BIOL CHEM, V267, P25722