GTP cyclohydrolase I mRNA induction and tetrahydrobiopterin synthesis in human endothelial cells

被引：29

作者：

Hattori, Y ^{[1
]}

Nakanishi, N ^{[1
]}

Kasai, K ^{[1
]}

Shimoda, SI ^{[1
]}

机构：

[1] MEIKAI UNIV,SCH DENT,DEPT BIOCHEM,SAKADO,SAITAMA 35002,JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1997年 / 1358卷 / 01期

关键词：

GTP cyclohydrolase I; tetrahydrobiopterin; endothelial cell; cytokine;

D O I：

10.1016/S0167-4889(97)00052-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The key role of tetrahydrobiopterin (BH4) in the synthesis of nitric oxide by human umbilical vein endothelial cells (HUVEC) has been demonstrated. We characterized the induction of BH4 synthesis in a cell line (ECV) derived from HUVEC and primary HUVEC. A significant induction of guanosine triphosphate cyclohydrolase I (GTPCH) mRNA was observed in response to TNF, IL-1 beta, and IFN gamma in ECV and HUVEC. The induction of GTPCH mRNA was abolished by actinomycin D. The cytokines led to an increased accumulation of BH4 in ECV. This effect was prevented by 2,4-diamino-6-hydroxypyrimidine, a selective inhibitor of GTPCH, as well as by actinomycin D and by cycloheximide. Results provide evidence for an increase in GTPCH activity and in BH4 levels in response to immunostimulants in human endothelial cells. (C) 1997 Elsevier Science B.V.

引用

页码：61 / 66

页数：6

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