Structure, measurement, and secretion of human glucagon-like peptide-2

被引：192

作者：

Hartmann, B

Johnsen, AH

Orskov, C

Adelhorst, K

Thim, L

Holst, JJ

机构：

[1] Univ Copenhagen, Panum Inst, Dept Med Physiol, DK-2200 Copenhagen N, Denmark

[2] Univ Copenhagen, Panum Inst, Dept Med Anat, DK-2200 Copenhagen, Denmark

[3] Rigshosp, Dept Clin Biochem, DK-2100 Copenhagen 0, Denmark

[4] Novo Nordisk AS, DK-2880 Bagsvaerd, Denmark

来源：

PEPTIDES | 2000年 / 21卷 / 01期

关键词：

proglucagon; radioimmunoassay; GLP-2; GLP-1; DPP-IV;

D O I：

10.1016/S0196-9781(99)00176-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

By using radioimmunoassays toward the cDNA-predicted amino acid sequence of human glucagon-like peptide-2, a peptide was isolated from extracts of human ileum. By mass spectrometry and Edman sequencing, this peptide was identified as human proglucagon 126-158. High-performance liquid chromatography analyses indicated that a similar immunoreactive peptide (iGLP-2) was present in human plasma. Human plasma concentrations of iGLP-2 were elevated 3- to 4-fold at 1 to 2 h after ingestion of 800 to 1200 kcal meals. (C) 2000 Elsevier Science Inc. All rights reserved.

引用

页码：73 / 80

页数：8

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