Kinesin-II is not essential for mitosis and cell growth in Chlamydomonas

被引:35
作者
Matsuura, K
Lefebvre, PA
Kamiya, R
Hirono, M [1 ]
机构
[1] Univ Tokyo, Dept Biol Sci, Bunkyo Ku, Tokyo 1130033, Japan
[2] Univ Minnesota, Dept Genet Cell Biol & Dev, St Paul, MN 55108 USA
[3] Univ Minnesota, Dept Plant Biol, St Paul, MN 55108 USA
[4] Natl Inst Basic Biol, Okazaki, Aichi 444, Japan
来源
CELL MOTILITY AND THE CYTOSKELETON | 2002年 / 52卷 / 04期
关键词
insertional mutagenesis; fla10 null mutant; microtubules; mitotic spindle;
D O I
10.1002/cm.10051
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The FLA10 gene product (Fla10p) in Chlamydomonas, a heterotrimeric kinesin-II, plays a crucial role in flagellar assembly as a motor protein driving intraflagellar transport. This protein has also been suggested to play a role in mitosis based on its localization to mitotic spindle. A role for Fla10p in mitosis has been difficult to test because to date only conditional (temperature-sensitive) mutant alleles were available, and it is not known whether these retain residual function for mitosis at the non-permissive temperature. In this report, we describe a null allele of fla10 produced by insertional mutagenesis. This mutant does not assemble flagella, but proliferates at a rate identical to that of wild type cells. Observation of microtubule organization in the cell body revealed that normal mitotic spindles are formed in dividing mutant cells. Thus, we conclude that FLA10 kinesin plays no significant roles in mitosis.
引用
收藏
页码:195 / 201
页数:7
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