Rab11 is associated with transferrin-containing recycling compartments in K562 cells

被引:93
作者
Green, EG
Ramm, E
Riley, NM
Spiro, DJ
Goldenring, JR
WesslingResnick, M
机构
[1] HARVARD UNIV, SCH PUBL HLTH, DEPT NUTR, BOSTON, MA 02115 USA
[2] MED COLL GEORGIA, INST MOL MED & GENET, AUGUSTA, GA 30912 USA
基金
美国国家卫生研究院;
关键词
Rab GTPases; K562; cloning; secretion;
D O I
10.1006/bbrc.1997.7520
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
3'RACE PCR was used La survey Rab transcripts synthesized by the human hematopoietic K562 cell line, Among the identified GTP-binding proteins, Rab11 was discovered, This result was unexpected since Rab11 previously had been found associated with polarized and secretary cells, Rab11 mRNA was abundant compared to that for other Rabs in K562 cells; protein levels represented 0.05-0.1% of total membrane protein. Localization of Rab11 using confocal immunofluoresence microscopy revealed extensive overlap with transferrin in recycling and/or exocytic compartments and suggests that Rab11 in non-polarized and non-secretory cells may play a role ina the trafficking and recycling of internalized proteins. (C) 1997 Academic Press.
引用
收藏
页码:612 / 616
页数:5
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