Raman microspectroscopic model of human breast tissue:: implications for breast cancer diagnosis in vivo

被引:246
作者
Shafer-Peltier, KE
Haka, AS
Fitzmaurice, M
Crowe, J
Myles, J
Dasari, RR
Feld, MS
机构
[1] MIT, George R Harrison Spect Lab, Cambridge, MA 02139 USA
[2] Univ Hosp Cleveland, Cleveland, OH 44106 USA
[3] Case Western Reserve Univ, Cleveland, OH 44106 USA
[4] Cleveland Clin Fdn, Cleveland, OH 44106 USA
[5] MIT, Laser Biomed Res Ctr, Cambridge, MA 02139 USA
关键词
D O I
10.1002/jrs.877
中图分类号
O433 [光谱学];
学科分类号
0703 ; 070302 ;
摘要
Raman spectroscopy has the potential to provide real-time, in situ diagnosis of breast cancer during needle biopsy or surgery via an optical fiber probe. Understanding the chemical/morphological basis of the Raman spectrum of breast tissue is a necessary step in developing Raman spectroscopy as a tool for in situ breast cancer diagnosis. To understand the relationship between the Raman spectrum of a sample of breast tissue and its disease state, near-infrared Raman spectroscopic images of human breast tissue were acquired using a confocal microscope. These images were then compared with phase contrast and hematoxylin- and eosin-stained images to develop a chemical/morphological model of breast tissue Raman spectra. This model fits macroscopic tissue spectra with a linear combination of basis spectra derived from spectra of the cell cytoplasm, cell nucleus, fat, beta-carotene, collagen, calcium hydroxyapatite, calcium oxalate dihydrate, cholesterol-like lipid deposits and water. Each basis spectrum represents data acquired from multiple patients and, when appropriate, from a variety of normal and diseased states. The model explains the spectral features of a range of normal and diseased breast tissue samples, including breast cancer. It can be used to relate the Raman spectrum of a breast tissue sample to diagnostic parameters used by pathologists. Copyright (C) 2002 John Wiley Sons, Ltd.
引用
收藏
页码:552 / 563
页数:12
相关论文
共 34 条
[1]  
ALINI M, 1991, CANCER RES, V51, P1443
[2]  
[Anonymous], 1991, Lasers in the Life Sciences
[3]  
BRENNAN JF, 1995, THESIS MIT CAMBRIDGE
[4]   Raman microspectroscopy of human coronary atherosclerosis: Biochemical assessment of cellular and extracellular morphologic structures in situ [J].
Buschman, HP ;
Deinum, G ;
Motz, JT ;
Fitzmaurice, M ;
Kramer, JR ;
van der Laarse, A ;
Bruschke, AV ;
Feld, MS .
CARDIOVASCULAR PATHOLOGY, 2001, 10 (02) :69-82
[5]   Diagnosis of human coronary atherosclerosis by morphology-based Raman spectroscopy [J].
Buschman, HP ;
Motz, JT ;
Deinum, G ;
Römer, TJ ;
Fitzmaurice, M ;
Kramer, JR ;
van der Laarse, A ;
Bruschke, AV ;
Feld, MS .
CARDIOVASCULAR PATHOLOGY, 2001, 10 (02) :59-68
[6]   FRACTIONAL ALLELIC IMBALANCE IN HUMAN BREAST-CANCER INCREASES WITH TETRAPLOIDIZATION AND CHROMOSOME LOSS [J].
CORNELISSE, CJ ;
KUIPERSDIJKSHOORN, N ;
VANVLIET, M ;
HERMANS, J ;
DEVILEE, P .
INTERNATIONAL JOURNAL OF CANCER, 1992, 50 (04) :544-548
[7]  
Cotran R., 1999, Robbins Pathologic Basis of Disease, V6th
[8]   Assessment of the size, position, and optical properties of breast tumors in vivo by noninvasive optical methods [J].
Fantini, S ;
Walker, SA ;
Franceschini, MA ;
Kaschke, M ;
Schlag, PM ;
Moesta, KT .
APPLIED OPTICS, 1998, 37 (10) :1982-1989
[9]   CHARACTERIZATION OF HUMAN BREAST BIOPSY SPECIMENS WITH NEAR-IR RAMAN-SPECTROSCOPY [J].
FRANK, CJ ;
REDD, DCB ;
GANSLER, TS ;
MCCREERY, RL .
ANALYTICAL CHEMISTRY, 1994, 66 (03) :319-326
[10]  
Gupta PK, 1997, LASER SURG MED, V21, P417, DOI 10.1002/(SICI)1096-9101(1997)21:5<417::AID-LSM2>3.0.CO