Possible involvement of the double-stranded RNA-binding core protein σA in the resistance of avian reovirus to interferon

被引:59
作者
Martínez-Costas, J
González-López, C
Vakharia, VN
Benavente, J [1 ]
机构
[1] Univ Santiago de Compostela, Fac Farm, Dept Bioquim & Biol Mol, Santiago De Compostela 15706, A Coruna, Spain
[2] Univ Maryland, Agr Biotechnol Ctr, Inst Biotechnol, College Pk, MD 20742 USA
[3] Univ Maryland, VA MD Reg Coll Vet Med, College Pk, MD 20742 USA
关键词
D O I
10.1128/JVI.74.3.1124-1131.2000
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Treatment of primary cultures of chicken embryo fibroblasts with a recombinant chicken alpha/beta interferon (rcIFN) induces an antiviral state that causes a strong inhibition of vaccinia virus and vesicular stomatitis virus replication but has no effect on avian reovirus S1133 replication, The fact that avian reovirus polypeptides are synthesized normally in rcIFN-treated cells prompted us to investigate whether this virus expresses factors that interfere with the activation and/or the activity of the IFN-induced, double-stranded RNA (dsRNA)-dependent enzymes. Our results demonstrate that extracts of avian-reovirus-infected cells, but not those of uninfected cells, are able to relieve the translation-inhibitory activity of dsRNA in reticulocyte lysates, by blocking the activation of the dsRNA-dependent enzymes. In addition, our results show that protein sigma A, an S1133 core polypeptide, binds to dsRNA in an irreversible manner and that clearing this protein from extracts of infected cells abolishes their protranslational capacity, Taken together, our results raise the interesting possibility that protein sigma A antagonizes the IFN-induced cellular response against avian reovirus by blocking the intracellular activation of enzyme pathways dependent an dsRNA as has been suggested for several other viral dsRNA-binding proteins.
引用
收藏
页码:1124 / 1131
页数:8
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共 67 条
[2]   DISTINCT PATTERNS OF IFN SENSITIVITY OBSERVED IN CELLS INFECTED WITH VACCINIA K3L(-) AND E3L(-) MUTANT VIRUSES [J].
BEATTIE, E ;
PAOLETTI, E ;
TARTAGLIA, J .
VIROLOGY, 1995, 210 (02) :254-263
[3]   MECHANISM OF INTERFERON ACTION - ACTIVATION OF THE HUMAN P1/ELF-2-ALPHA-PROTEIN KINASE BY INDIVIDUAL REOVIRUS S-CLASS MESSENGER-RNAS - S1 MESSENGER-RNA IS A POTENT ACTIVATOR RELATIVE TO S4 MESSENGER-RNA [J].
BISCHOFF, JR ;
SAMUEL, CE .
VIROLOGY, 1989, 172 (01) :106-115
[4]   VIRUS-MEDIATED INHIBITION OF THE PPP(A2'P) A SYSTEM AND ITS PREVENTION BY INTERFERON-N [J].
CAYLEY, PJ ;
KNIGHT, M ;
KERR, IM .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1982, 104 (02) :376-382
[5]   ACTIVATION OF THE PPP(A2'P)NA SYSTEM IN INTERFERON-TREATED, HERPES-SIMPLEX VIRUS-INFECTED CELLS AND EVIDENCE FOR NOVEL INHIBITORS OF THE PPP(A2'P)NA-DEPENDENT RNASE [J].
CAYLEY, PJ ;
DAVIES, JA ;
MCCULLAGH, KG ;
KERR, IM .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1984, 143 (01) :165-174
[6]   IDENTIFICATION OF A CONSERVED MOTIF THAT IS NECESSARY FOR BINDING OF THE VACCINIA VIRUS E3L GENE-PRODUCTS TO DOUBLE-STRANDED-RNA [J].
CHANG, HW ;
JACOBS, BL .
VIROLOGY, 1993, 194 (02) :537-547
[7]   THE E3L GENE OF VACCINIA VIRUS ENCODES AN INHIBITOR OF THE INTERFERON-INDUCED, DOUBLE-STRANDED RNA-DEPENDENT PROTEIN-KINASE [J].
CHANG, HW ;
WATSON, JC ;
JACOBS, BL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (11) :4825-4829
[8]   PKR - PROPOSED NOMENCLATURE FOR THE RNA-DEPENDENT PROTEIN-KINASE INDUCED BY INTERFERON [J].
CLEMENS, MJ ;
HERSHEY, JWB ;
HOVANESSIAN, AC ;
JACOBS, BC ;
KATZE, MG ;
KAUFMAN, RJ ;
LENGYEL, P ;
SAMUEL, CE ;
SEN, GC ;
WILLIAMS, BRG .
JOURNAL OF INTERFERON RESEARCH, 1993, 13 (03) :241-241
[9]   PKR - A protein kinase regulated by double-stranded RNA [J].
Clemens, MJ .
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 1997, 29 (07) :945-949
[10]   The double-stranded RNA-dependent protein kinase PKR: Structure and function [J].
Clemens, MJ ;
Elia, A .
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH, 1997, 17 (09) :503-524