A biosensor for Escherichia coli based on a potentiometric alternating biosensing (PAB) transducer

被引:27
作者
Ercole, C [1 ]
Del Gallo, M
Pantalone, M
Santucci, S
Mosiello, L
Laconi, C
Lepidi, A
机构
[1] Univ Aquila, Dept Basic & Appl Biol, I-67010 Coppito, Italy
[2] Univ Aquila, Dept Phys, I-67010 Coppito, Italy
[3] Univ Aquila, Unita INFM, I-67010 Coppito, Italy
[4] ENEA Casaccia, I-00060 Rome, Italy
来源
SENSORS AND ACTUATORS B-CHEMICAL | 2002年 / 83卷 / 1-3期
关键词
biosensor; E; coli; water pollution; light addressable potentiometric sensor (LAPS); immunoassay;
D O I
10.1016/S0925-4005(01)01027-9
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
In the present paper, we describe a biosensor for the detection of bacterial contamination in drinking water based on an immunoassay test. The biological component was made by a rabbit polyclonal antibody obtained utilising an environmental strain of Escherichia coli, DH5alpha. This strain, showed a capsular protein pattern typical of this species. Cells present in water samples were detected by a complete potentiometric alternating biosensor (PAB). The PAB system consists of a measuring chamber, acquisition and driving electronics and the appropriate software: the apparatus is computer controlled in order to obtain on-line acquisitions and recording of data. The transducer principle is based on a light addressable potentiometric sensor (LAPS) technology which, in our case, reveals the production of NH3 by a urease-E. coli antibody conjugate. The proposed system appears to be very sensitive and fast, in comparison with conventional methods: concentrations of 10 cells per ml were detected in an assay time of ca. 1.5 h. No signals were detected from other potential water polluting bacteria, such as Pseudomonas marina and Sphaerotilus natans and also from bacteria such as Klebsiella oxytoca phyletically related to E. coli. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:48 / 52
页数:5
相关论文
共 7 条
[1]  
*AAVV, 1998, BIOL MAN, P77
[2]   PAB - A NEWLY DESIGNED POTENTIOMETRIC ALTERNATING BIOSENSOR SYSTEM [J].
ADAMI, M ;
SARTORE, M ;
NICOLINI, C .
BIOSENSORS & BIOELECTRONICS, 1995, 10 (1-2) :155-167
[3]   Use of a light-addressable potentiometric sensor for the detection of Escherichia coli O157:H7 [J].
Gehring, AG ;
Patterson, DL ;
Tu, SI .
ANALYTICAL BIOCHEMISTRY, 1998, 258 (02) :293-298
[4]  
Harlow E, 1988, ANTIBODIES LAB MANUA
[5]   Immunoenzymatic application of a redox potential biosensor [J].
Piras, L ;
Adami, M ;
Fenu, S ;
Dovis, M ;
Nicolini, C .
ANALYTICA CHIMICA ACTA, 1996, 335 (1-2) :127-135
[6]   Development of a biosensor for E-coli based on a flexural plate wave (FPW) transducer [J].
Pyun, JC ;
Beutel, H ;
Meyer, JU ;
Ruf, HH .
BIOSENSORS & BIOELECTRONICS, 1998, 13 (7-8) :839-845
[7]  
SEELEY HW, 1997, LAB MICROBIOLOGIA, P70