The 26 nudix hydrolases of Bacillus cereus, a close relative of Bacillus anthracis

被引：48

作者：

Xu, WL

Dunn, CA

Jones, CR

D'Souza, G

Bessman, MJ ^{[1
]}

机构：

[1] Johns Hopkins Univ, Dept Biol, Baltimore, MD 21218 USA

[2] Johns Hopkins Univ, McCollum Pratt Inst, Baltimore, MD 21218 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2004年 / 279卷 / 23期

关键词：

D O I：

10.1074/jbc.M403272200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The genome of Bacillus cereus contains 26 Nudix hydrolase genes, second only to its closest relative, Bacillus anthracis which has 30. All 26 genes have been cloned, 25 have been expressed, and 21 produced soluble proteins suitable for analysis. Substrates for 16 of the enzymes were identified; these included ADP-ribose, diadenosine polyphosphates, sugar nucleotides, and deoxynucleoside triphosphates. One of the enzymes was a CDP-choline pyrophosphatase, the first Nudix hydrolase active on this substrate. Furthermore, as a result of this and previous work we have identified a new subfamily of the Nudix hydrolase superfamily recognizable by a specific amino acid motif outside of the Nudix box.

引用

页码：24861 / 24865

页数：5

共 18 条

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