Indirect evidence of direct electron communication between the active site of galactose oxidase and a graphite electrode

被引:14
作者
Tkac, J
Vostiar, I
Gemeiner, P
Sturdik, E
机构
[1] Slovak Acad Sci, Inst Chem, SK-84238 Bratislava, Slovakia
[2] Slovak Univ Technol Bratislava, Fac Chem & Food Technol, Dept Biochem Technol, SK-81237 Bratislava, Slovakia
关键词
biosensor; galactose oxidase; direct electron transfer; copper oxidase;
D O I
10.1016/S1567-5394(02)00043-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Bi-enzymatic biosensor based on galactose oxidase (GalOD) and horseradish peroxidase (HRP) using ferrocene as an efficient mediator was constructed. When a dependence of a working potential on the sensor performance was examined, an unusual behaviour was observed. With increasing of an applied working potential a lower concentration of substrate to attain full linear range was needed. A fully linear dependence from the first substrate addition was observed at and above the working potential of 150 mV. This activation of the biosensor response by an applied working potential very well corresponds with a formal potential of GalOD (156 mV). When a membrane prevented GalOD access to the electrode surface was applied, no activation effect of a working potential on the sensor performance was observed. Thus, it can be assumed that direct electron communication between GalOD and the electrode occurred. (C) 2002 Elsevier Science B.V All rights reserved.
引用
收藏
页码:23 / 25
页数:3
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