Formulation of a protein-free medium for human assisted reproduction

被引:15
作者
Ali, J
Shahata, MAM
Al-Natsha, SD
机构
[1] Hamad Med Corp, IVF Unit, Womens Hosp, Doha, Qatar
[2] JA Global Pty Ltd, Curtin, ACT 2605, Australia
关键词
co-culture; embryos; live birth; micro-droplet; protein-free medium;
D O I
10.1093/humrep/15.1.145
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
The optimal concentrations of individual amino acids, antioxidants, vitamins, osmolytes and energy sources were determined using a 1-cell Swiss outbred (SO) and or F-1 [(CBA x C57BL/6J) x SO] mouse assay in Earle's balanced salt solution containing bovine serum albumin. Based on the findings of these experiments, a number of media were formulated. Of these, the medium showing optimal embryo development and a significantly higher blastocyst hatching rate was investigated further A protein-free medium (ART-7) was formulated and assessed using 1-, 2- and 4-cell SO mouse embryos. The generation of viable human embryos in the ART-7 series of media in micro- and ultra micro-droplet culture under oil with and without cumulus co-culture following intracytoplasmic sperm injection (ICSI) was investigated. The quality of sibling day 2 human embryos generated in the ART-7 media series was statistically comparable to or better than control embryos. The ART-7 medium was not toxic to human spermatozoa, Fertilization by conventional IVF and subsequent embryo development was not affected, A clinical trial of ICSI-derived embryos generated in the protein-free medium, with and without cumulus co-culture, has resulted in clinical pregnancies (10 of 20 transfers) of which two have proceeded to term, and the remaining patients are in various stages of pregnancy.
引用
收藏
页码:145 / 156
页数:12
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