The validation of self-reported smoking status by analysing cotinine levels in stimulated and unstimulated saliva, serum and urine

被引:106
作者
Binnie, V
McHugh, S
Macpherson, L
Borland, B
Moir, K
Malik, K
机构
[1] Glasgow Dent Sch, Glasgow G2 3JZ, Lanark, Scotland
[2] Gartnavel Royal Hosp, N Glasgow NHS Trust, Dept Biochem, Glasgow, Lanark, Scotland
[3] Birmingham Dent Sch, Birmingham, W Midlands, England
[4] Royal Dent Hosp Melbourne, Melbourne, Vic, Australia
关键词
tobacco; cotinine; cross-sectional study;
D O I
10.1111/j.1601-0825.2004.01018.x
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
OBJECTIVES: Cotinine, a nicotine metabolite, can be used to measure exposure to tobacco smoke. The aim of this study was to compare cotinine levels in different biological fluids collected from both smokers and non-smokers and to relate the findings to self-reported smoking status. Data were also collected concerning the acceptability of the differing methods of sample collection. MATERIAL AND METHOD: Patients recruited to the study were asked to provide samples of urine, blood and saliva (both stimulated and unstimulated). Data collected from patients by questionnaire included information on smoking behaviour such as daily number of cigarettes and environmental exposure to smoke. After the sample collection, patients were asked to rate the acceptability of each sampling method. Samples were analysed using enzyme immunoassay (EIA) kits. RESULTS: In total, 80 patients participated, with 49 being smokers and 31 being non-smokers. There was clear differentiation between smokers and non-smokers (P < 0.001) for all the different samples in terms of cotinine. A significant relationship was seen between cotinine and daily number of cigarettes for both salivas and urine (all P < 0.001) but not for serum. Participants found serum and urine collection methodologies 'very acceptable' (67 and 66%, respectively) whereas 9% found collection of stimulated saliva 'not at all acceptable'. CONCLUSION: Cotinine, whatever the collection method and analysed by EIA kits, shows good differentiation between smokers and non-smokers. Salivary samples have the advantage of being non-invasive, although collection methodology is important, as cotinine levels may vary.
引用
收藏
页码:287 / 293
页数:7
相关论文
共 26 条
[1]  
Bajekal M., 2003, Health Survey for England (2001)
[2]   INFLUENCE OF CIGARETTE, PIPE, AND CIGAR SMOKING, REMOVABLE PARTIAL DENTURES, AND AGE ON ORAL LEUKOPLAKIA [J].
BARIC, JM ;
ALMAN, JE ;
FELDMAN, RS ;
CHAUNCEY, HH .
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY, 1982, 54 (04) :424-429
[3]   Comparison of serum and salivary cotinine measurements by a sensitive high-performance liquid chromatography-tandem mass spectrometry method as an indicator of exposure to tobacco smoke among smokers and nonsmokers [J].
Bernert, JT ;
McGuffey, JE ;
Morrison, MA ;
Pirkle, JL .
JOURNAL OF ANALYTICAL TOXICOLOGY, 2000, 24 (05) :333-339
[4]  
CALLUM C, 1998, UK SMOKING EPIDEMIC
[5]   Smoking cessation counselling - A role for the dental profession? [J].
Chestnutt, IG ;
Binnie, VI .
BRITISH DENTAL JOURNAL, 1995, 179 (11-12) :411-415
[6]   MEASUREMENT OF CURRENT EXPOSURE TO ENVIRONMENTAL TOBACCO-SMOKE [J].
CUMMINGS, KM ;
MARKELLO, SJ ;
MAHONEY, M ;
BHARGAVA, AK ;
MCELROY, PD ;
MARSHALL, JR .
ARCHIVES OF ENVIRONMENTAL HEALTH, 1990, 45 (02) :74-79
[7]  
Etter JF, 2000, AM J EPIDEMIOL, V151, P251
[8]   A REVIEW OF THE USE OF SALIVA COTININE AS A MARKER OF TOBACCO-SMOKE EXPOSURE [J].
ETZEL, RA .
PREVENTIVE MEDICINE, 1990, 19 (02) :190-197
[9]   A RAPID GAS-LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF COTININE AND NICOTINE IN BIOLOGICAL-FLUIDS [J].
FEYERABEND, C ;
RUSSELL, MAH .
JOURNAL OF PHARMACY AND PHARMACOLOGY, 1990, 42 (06) :450-452
[10]   STABILITY OF SALIVARY COTININE SENT THROUGH THE UNITED-STATES MAIL FOR VERIFICATION OF SMOKING STATUS [J].
GREELEY, DA ;
VALOIS, RF ;
BERNSTEIN, DA .
ADDICTIVE BEHAVIORS, 1992, 17 (03) :291-296