Flavonoids from Astragalus membranaceus and their inhibitory effects on LPS-stimulated pro-inflammatory cytokine production in bone marrow-derived dendritic cells

被引：136

作者：

Li, Wei ^{[1
]}

Sun, Ya Nan ^{[1
]}

Yan, Xi Tao ^{[1
]}

Yang, Seo Young ^{[1
]}

Kim, Sohyun ^{[2
,3
,4
]}

Lee, Young Mi ^{[5
]}

Koh, Young-Sang ^{[2
,3
,4
]}

Kim, Young Ho ^{[1
]}

机构：

[1] Chungnam Natl Univ, Coll Pharm, Taejon 305764, South Korea

[2] Jeju Natl Univ, Sch Med, Cheju 690756, South Korea

[3] Jeju Natl Univ, Brain Korea Program 21, Cheju 690756, South Korea

[4] Jeju Natl Univ, Inst Med Sci, Cheju 690756, South Korea

[5] Wonkwang Univ, Coll Pharm, Dept Oriental Pharm, Iksan 570749, Jeonbuk, South Korea

来源：

ARCHIVES OF PHARMACAL RESEARCH | 2014年 / 37卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

Astragalus membranaceus; Leguminosae; Flavonoid; Anti-inflammatory; DISEASE; RADIX; LIQUIRITIGENIN; INTERLEUKIN-6; CONSTITUENTS; EXPRESSION; SECRETION; LEAVES;

D O I：

10.1007/s12272-013-0174-7

中图分类号：

R914 [药物化学];

学科分类号：

100705 [微生物与生化药学];

摘要：

Radix Astragali (Astragalus membranaceus) is an important traditional Chinese medicine that is widely used as a tonic to enhance the body's natural defense mechanisms. In this phytochemical study, 12 flavonoids, isoliquiritigenin (1), liquiritigenin (2), calycosin (3), calycosin 7-O-beta-d-glucoside (4), formononetin (5), formononetin 7-O-beta-d-glucoside (6), daidzein (7), daidzein 7-O-beta-d-glucoside (8), methylnissolin (9), methylnissolin 3-O-beta-d-glucoside (10), isomucronulatol (11), and isomucronulatol 7-O-beta-d-glucoside (12), were isolated from the roots of A. membranaceus. Their structures were elucidated by comparing spectroscopic data with reported values. The effects of the isolated compounds on lipopolysaccharide (LPS)-stimulated bone marrow-derived dendritic cells were investigated. Compounds 1 and 2 exhibited significant inhibitory effects on LPS-induced IL-6 and IL-12 p40 production, with IC50 values ranging from 2.7 to 6.1 mu M. Compound 1 also showed a moderate inhibitory effect on LPS-stimulated production of TNF-alpha with an IC50 value of 20.1 mu M. Further studies of the potential anti-inflammatory effects and benefits of flavonoids from A. membranaceus are warranted.

引用

页码：186 / 192

页数：7

共 35 条

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